Vaginal CD4+ T cells express high levels of CCR5 and are rapidly depleted in simian immunodeficiency virus infection

被引:101
作者
Veazey, RS [1 ]
Marx, PA [1 ]
Lackner, AA [1 ]
机构
[1] Tulane Univ, Hlth Sci Ctr, Tulane Natl Primate Res Ctr, Covington, LA 70433 USA
关键词
D O I
10.1086/368386
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Worldwide, the majority of human immunodeficiency virus-1 cases occur through heterosexual transmission, yet little is known regarding the phenotype of CD4(+) T cells in the vaginal mucosa. In the present study, lymphocytes were compared from the lymph nodes, blood, and vagina from uninfected and simian immunodeficiency virus (SIV)-infected macaques. In mature female macaques, 54%-67% of the vaginal CD4(+) T cells expressed C chemokine receptor 5 (CCR5), whereas 84%-99% coexpressed CXC chemokine receptor 4. In contrast, only 4.4%-14.8% of peripheral blood and 2.4%-13% of lymph-node CD4(+) T cells coexpressed CCR5. Moreover, CCR5 mean channel fluorescence was significantly higher on CD4 cells from the vagina, compared with those from blood. In macaques intravenously infected with SIV, rapid depletion of CD4(+) T cells was observed in the vagina, particularly among the CCR5(+) CD4(+) subset. This demonstrates that large numbers of CD4(+) T cells expressing high levels of CCR5 reside within the vagina and that these cells are preferentially targeted for elimination by SIV infection.
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收藏
页码:769 / 776
页数:8
相关论文
共 21 条
[1]   The role of DC-SIGN and DC-SIGNR in HIV and SIV attachment, infection, and transmission [J].
Baribaud, F ;
Pöhlmann, S ;
Doms, RW .
VIROLOGY, 2001, 286 (01) :1-6
[2]   NECROPSY FINDINGS IN RHESUS-MONKEYS EXPERIMENTALLY INFECTED WITH CULTURED SIMIAN IMMUNODEFICIENCY VIRUS (SIV)-DELTA [J].
BASKIN, GB ;
MURPHEYCORB, M ;
WATSON, EA ;
MARTIN, LN .
VETERINARY PATHOLOGY, 1988, 25 (06) :456-467
[3]   The dendritic cell-T cell milieu of the lymphoid tissue of the tonsil provides a locale in which SIV can reside and propagate at chronic stages of infection [J].
Hu, JJ ;
Miller, CJ ;
O'Doherty, U ;
Marx, PA ;
Pope, M .
AIDS RESEARCH AND HUMAN RETROVIRUSES, 1999, 15 (14) :1305-1314
[4]   Simian immunodeficiency virus rapidly penetrates the cervicovaginal mucosa after intravaginal inoculation and infects intraepithelial dendritic cells [J].
Hu, JJ ;
Gardner, MB ;
Miller, CJ .
JOURNAL OF VIROLOGY, 2000, 74 (13) :6087-6095
[5]   Dendritic cells from skin and blood of macaques both promote SIV replication with T cells from different anatomical sites [J].
Ignatius, R ;
Isdell, F ;
O'Doherty, U ;
Pope, M .
JOURNAL OF MEDICAL PRIMATOLOGY, 1998, 27 (2-3) :121-128
[6]  
KELL PD, 1992, J ROY SOC MED, V85, P706
[7]   The number and distribution of immune cells in the cervicovaginal mucosa remain constant throughout the menstrual cycle of rhesus macaques [J].
Ma, ZM ;
Lü, FX ;
Torten, M ;
Miller, CJ .
CLINICAL IMMUNOLOGY, 2001, 100 (02) :240-249
[8]   Progesterone implants enhance SIV vaginal transmission and early virus load [J].
Marx, PA ;
Spira, AI ;
Gettie, A ;
Dailey, PJ ;
Veazey, RS ;
Lackner, AA ;
Mahoney, CJ ;
Miller, CJ ;
Claypool, LE ;
Ho, DD ;
Alexander, NJ .
NATURE MEDICINE, 1996, 2 (10) :1084-1089
[9]   The decreased replicative capacity of simian immunodeficiency virus SIVmac239Δnef is manifest in cultures of immature dendritic cells and T cells [J].
Messmer, D ;
Ignatius, R ;
Santiseban, C ;
Steinman, RM ;
Pope, M .
JOURNAL OF VIROLOGY, 2000, 74 (05) :2406-2413
[10]  
Miller C. J., 1992, Laboratory Investigation, V68, P129