Description of a novel HLA-B allele, B*5613, identified during HLA-typing using sequence-specific oligonucleotide hybridization and sequence-specific amplification

被引：5

作者：

Hoppe, B

Heymann, GA

Schoenemann, C

Nagy, M

Kiesewetter, H

Salama, A

机构：

[1] Charite Univ Med Berlin, Inst Transfus Med, D-13353 Berlin, Germany

[2] Charite Univ Med Berlin, Inst Legal Med, D-13353 Berlin, Germany

来源：

TISSUE ANTIGENS | 2004年 / 64卷 / 05期

关键词：

allele-specific amplification; DNA sequencing; SSO; SSP;

D O I：

10.1111/j.1399-0039.2004.00306.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Here, we report on the characterization of a novel human leukocyte antigen (HLA)-B allele, B*5613. The allele was identified in an adult male from North Africa who was suffering from sickle cell anemia. HLA-B*5613 most closely matches to B*5601 differing only by a substitution of three nucleotides of codon 180. Due to this substitution, low-resolution HLA-typing using sequence-specific oligonucleotide hybridization or amplification using sequence-specific primers gave inconclusive results. DNA sequencing confirmed a variation of codon 180 (CTG-->GAC) resulting in an amino acid substitution Leu156Asp.

引用

页码：616 / 618

页数：3

共 4 条

[1] The structure of HLA-B8 complexed to an immunodominant viral determinant: Peptide-induced conformational changes and a mode of MHC class I dimerization [J].