Neurotrophic effect of basic fibroblast growth factor is mediated by the p42/p44 mitogen-activated protein kinase cascade in cultured rat cortical neurons

被引：45

作者：

Abe, K ^{[1
]}

Saito, H ^{[1
]}

机构：

[1] Univ Tokyo, Fac Pharmaceut Sci, Dept Clin Pharmacol, Bunkyo Ku, Tokyo 1130033, Japan

来源：

DEVELOPMENTAL BRAIN RESEARCH | 2000年 / 122卷 / 01期

关键词：

basic fibroblast growth factor; mitogen-activated protein kinase; neuronal survival; rat cortical neuron; culture;

D O I：

10.1016/S0165-3806(00)00054-7

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Basic fibroblast growth factor (bFGF) has been reported to support the survival of brain neurons. In the present study, we investigated whether the neurotrophic effect of bFGF is mediated by the mitogen-activated protein kinase (MAPK) cascade in cultured rat cerebral cortical neurons. Recombinant human bFGF (0.1-10 ng/ml) induced phosphorylation of p44/42 MAPK (ERK1/2) in a concentration and time-dependent manner. bFGF-induced ERK1/2 phosphorylation and promotion of neuronal survival were both blocked by U0126 and PD98059, inhibitors of the MAPK-activating enzyme MEK. These results suggest that the MEK/ERK signal transduction cascade is involved in the neurotrophic effect of bFGF. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：81 / 85

页数：5

共 24 条

[1] The p44/42 mitogen-activated protein kinase cascade is involved in the induction and maintenance of astrocyte stellation mediated by protein kinase C
Abe, K
Saito, H
[J]. NEUROSCIENCE RESEARCH, 2000, 36 (03) : 251 - 257
[2] EFFECTS OF RECOMBINANT HUMAN BASIC FIBROBLAST GROWTH-FACTOR AND ITS MODIFIED PROTEIN CS23 ON SURVIVAL OF PRIMARY CULTURED NEURONS FROM VARIOUS REGIONS OF FETAL-RAT BRAIN
ABE, K
TAKAYANAGI, M
SAITO, H
[J]. JAPANESE JOURNAL OF PHARMACOLOGY, 1990, 53 (02) : 221 - 227
[3] Abe K, 1996, J NEUROCHEM, V67, P2074
[4] BASIC FIBROBLAST GROWTH-FACTOR PREVENTS DEATH OF LESIONED CHOLINERGIC NEURONS INVIVO
ANDERSON, KJ
DAM, D
LEE, S
COTMAN, CW
[J]. NATURE, 1988, 332 (6162) : 360 - 361
[5] Corbit KC, 1999, MOL CELL BIOL, V19, P4209
[6] A SYNTHETIC INHIBITOR OF THE MITOGEN-ACTIVATED PROTEIN-KINASE CASCADE
DUDLEY, DT
PANG, L
DECKER, SJ
BRIDGES, AJ
SALTIEL, AR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (17) : 7686 - 7689
[7] Identification of a novel inhibitor of mitogen-activated protein kinase kinase
Favata, MF
Horiuchi, KY
Manos, EJ
Daulerio, AJ
Stradley, DA
Feeser, WS
Van Dyk, DE
Pitts, WJ
Earl, RA
Hobbs, F
Copeland, RA
Magolda, RL
Scherle, PA
Trzaskos, JM
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (29) : 18623 - 18632
[8] FIBROBLAST GROWTH-FACTOR
GOSPODAROWICZ, D
NEUFELD, G
SCHWEIGERER, L
[J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1986, 46 (03) : 187 - 204
[9] ISOLATION OF BRAIN FIBROBLAST GROWTH-FACTOR BY HEPARIN-SEPHAROSE AFFINITY-CHROMATOGRAPHY - IDENTITY WITH PITUITARY FIBROBLAST GROWTH-FACTOR
GOSPODAROWICZ, D
CHENG, J
LUI, GM
BAIRD, A
BOHLENT, P
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (22): : 6963 - 6967
[10] Phosphatidylinositol-3 kinase dependent MAP kinase activation via p21ras in endothelial cells exposed to cyclic strain
Ikeda, M
Kito, H
Sumpio, BE
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 257 (03) : 668 - 671

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