Persistence of hepatitis A virus in oysters

被引：49

作者：

Kingsley, DH ^{[1
]}

Richards, GP ^{[1
]}

机构：

[1] Delaware State Univ, WW Baker Ctr, USDA ARS, Microbial Food Safety Res Unit, Dover, DE 19901 USA

来源：

JOURNAL OF FOOD PROTECTION | 2003年 / 66卷 / 02期

关键词：

D O I：

10.4315/0362-028X-66.2.331

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We investigated the ability of hepatitis A virus (HAV) to persist for up to 6 weeks in Eastern oysters (Crassostrea virginica). Viral RNA was detected by reverse transcription-polymerase chain reaction 6 weeks after 16 h of exposure to 90,000 PFU (180 PFU/ml of seawater) of HAV. Assaying for infectious virus in oysters that received a daily feeding of phytoplankton recovered 3,800, 650, and 500 PFU of HAV 1, 2, and 3 weeks after contamination with 90,000 PFU of HAV, respectively. However, no infectious HAV was isolated from oysters 4, 5, or 6 weeks after contamination. These results support the position that shellfish depuration is insufficient for the complete removal of infectious viruses. Extended relay times (in excess of 4 weeks) may be required to produce virologically safe shellfish.

引用

页码：331 / 334

页数：4

共 20 条

[1]

[Anonymous], MICROBIOLOGY MARINE

[2]

Arnal C, 1998, ZBL HYG UMWELTMED, V201, P279

[3] DIFFERENTIAL ACCUMULATION AND DEPURATION OF HUMAN ENTERIC VIRUSES BY MUSSELS [J].