Conservation of microsatellites among tropical trees (Leguminosae)

被引:87
作者
Dayanandan, S [1 ]
Bawa, KS [1 ]
Kesseli, R [1 ]
机构
[1] Univ Massachusetts, Dept Biol, Boston, MA 02125 USA
关键词
Ingeae; Mimosoideae; phylogeny; Pithecellobium; rbcL; simple sequence repeat;
D O I
10.2307/2446463
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Although microsatellites or simple sequence repeats (SSRs) have become a popular tool in genetic mapping and gene flow studies, their utility is limited due to paucity of information about DNA sequences in plants. We tested the utility of microsatellite markers characterized for the tropical tree Pithecellobium elegans as a genetic tool for related species. The results indicate that SSR loci are conserved among closely related species, and SSR primers developed for P. elegans could be successfully used as a genetic tool in several species of the tribe Ingeae. This study indicates that there is high potential for the transfer of SSR markers among closely related taxa, circumventing laborious cloning and screening procedures involved in characterizing SSR loci for many species.
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页码:1658 / 1663
页数:6
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