Does the preoptic anterior hypothalamus receive thermoafferent information?

The preoptic anterior hypothalamus (POAH) is considered the thermointegrative center of the mammalian brain. Studies on anesthetized and unanesthetized animals have demonstrated neurons in the POAH that respond to changes in both POAH temperature (T-POAH) and skin temperature (T-s). In these studies, however, electroencephalographic (EEG) activity was not monitored. Recent work has revealed the potential for arousal state selectivity of neurons combined with thermal influences on arousal state to create the appearance that cells are thermosensitive or thermoresponsive when in fact they may not be responding directly to temperature or to thermoafferent input. It is therefore necessary to reexamine the influence of central and peripheral temperature on POAH cells. In the present study, 66 POAH cells were recorded from urethan-anesthetized rats while EEG, T-POAH, and T-s were monitored. Seventy-five percent (41 of 55) of the cells were EEG state responsive; 22% (6 of 27) were T-POAH sensitive; and 33% (19 of 58) appeared to be T-s responsive. However, when EEG state changes mere taken into account, none of the cells that appeared to be T-s responsive were responding to T-s within any uniform EEG state. All changes in their firing rates were associated with EEG state changes. This study raises a question as to whether or not peripheral temperature information is integrated in the POAH. Consideration should be given to the possibility that T-s information is integrated lower in the neuroaxis. Monitoring EEG is essential in studies attempting to characterize the integrative properties of POAH neurons of anesthetized or unanesthetized animals. This caveat applies not just to thermoregulatory studies but to investigations of other integrative functions of the hypothalamus and many other brain regions as well.