Precise determinations of C and D periods by flow cytometry in Escherichia coli K-12 and B/r

被引:83
作者
Michelsen, O
de Mattos, MJT
Jensen, PR
Hansen, FG
机构
[1] Tech Univ Denmark DTU, Bioctr DTU, Sect Mol Microbiol, DK-2800 Lyngby, Denmark
[2] Univ Amsterdam, Bioctr Amsterdam, EC Slater Inst, Dept Microbiol, NL-1018 WS Amsterdam, Netherlands
来源
MICROBIOLOGY-SGM | 2003年 / 149卷
关键词
D O I
10.1099/mic.0.26058-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The C and D cell cycle periods of seven Escherichia coli K-12 strains and three E, coli B/r strains were determined by computer simulation of DNA histograms obtained by flow cytometry of batch cultures grown at several different generation times. To obtain longer generation times two of the K-12 strains were cultivated at several different dilution rates in glucose-limited chemostats. The replication period (C period) was found to be similar in K-12 and B/r strains grown at similar generation times. At generation times below 60 min the C period was constant; above 60 min it increased linearly with increasing generation time. The period from termination of replication to cell division (D period) was more variable. It was much shorter in B/r than in K-12 strains. Like the C period it was relatively constant at generation times below 60 min and it increased with increasing generation times at longer generation times. In glucose-limited chemostats good correlation was found between D periods and generation times, whereas batch cultures exhibited carbon-source-dependent variations. Chemostat cultures showed cell cycle variations very similar to those obtained in batch cultures. These flow cytometric determinations of cell cycle periods confirm earlier determinations of the C period and establish that the D period also varies with generation time in slowly growing cultures. In addition they extend the range of growth rates at which cell cycle periods have been determined in E. coli K-12.
引用
收藏
页码:1001 / 1010
页数:10
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