Spatial distribution of 16S rRNA levels from uncultured acidobacteria in soil

被引：18

作者：

Felske, A ^{[1
]}

de Vos, WM ^{[1
]}

Akkermans, ADL ^{[1
]}

机构：

[1] Wageningen Univ, Microbiol Lab, Wageningen, Netherlands

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2000年 / 31卷 / 02期

关键词：

D O I：

10.1046/j.1365-2672.2000.00780.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The activity of uncultured acidobacteria was monitored in Dutch grassland soils by quantifying their ribosomes. These bacteria were detectable by five different 16S rRNA RT-PCR products in temperature gradient gel electrophoresis fingerprints. The ribosomes in surface soil samples were quantified with multiple competitive RT-PCR along a 1.5-km transect through the grassland. in total, the five members of the acidobacteria were estimated to contribute 4 x 10(10) to 1 x 10(11) ribosomes g soil(-1), representing 7-14% of all bacterial ribosomes. These results indicate that ribosomes from acidobacteria an continuously present and abundant in soil and might contribute significantly to microbial activity in soil.

引用

页码：118 / 122

页数：5

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