Protection against ischemic brain damage by GDNF affecting cell survival and death signals

被引：51

作者：

Jin, G ^{[1
]}

Omori, N ^{[1
]}

Li, F ^{[1
]}

Nagano, I ^{[1
]}

Manabe, Y ^{[1
]}

Shoji, M ^{[1
]}

Abe, K ^{[1
]}

机构：

[1] Okayama Univ, Grad Sch Med & Dent, Dept Neurol, Okayama 7008558, Japan

来源：

NEUROLOGICAL RESEARCH | 2003年 / 25卷 / 03期

关键词：

Akt; caspase-3; caspase-9; glial cell line-derived neurotrophic factor (GDNF); ischemia; rat;

D O I：

10.1179/016164103101201454

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Neuroprotective effects of glial cell line-derived neurotrophic factor (GDNF) on cell survival and death signals were investigated after 90 min of transient middle cerebral artery occlusion (MCAO) in rats. Immunoreactivities of phosphorylated Akt (p-Akt), cleaved caspase-9 (c-cas9), and -3 (c-cas3) increased after the reperfusion in the penumbra in vehicle group with peaks at 3 h, S h, and 1 day, respectively. Topical application of GDNF (6.8 mug/9 mul) on brain surface potentiated and prolonged p-Akt activation, but suppressed activation of the caspases, and reduced the number of terminal deoxynucleotidyl transferase-mediated dUDP-biotin in situ nick labeling (TUNEL) positive cells: These results suggest that GDNF plays a protective role against ischemic injury by controlling the balance between Akt pathway and caspase cascades.

引用

页码：249 / 253

页数：5

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