Quantitative real-time PCR: a powerful ally in cancer research

被引:141
作者
Mocellin, S
Rossi, CR
Pilatil, P
Nitti, D
Marincola, FM
机构
[1] Univ Padua, Dept Oncol & Surg Sci, Surg Branch, I-35128 Padua, Italy
[2] NIH, Immunogenet Lab, Dept Transfus Med, Ctr Clin, Bethesda, MD 20814 USA
关键词
D O I
10.1016/S1471-4914(03)00047-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this era of the Human Genome Project, quantitation of gene expression in tumor or host cells is of paramount importance for investigating the gene patterns responsible for cancer development, progression and response or resistance to treatment. Quantitative real-time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that supports its use as a routine bioinstrumentation for gene level measurement. Several applications have already been implemented in the field of cancer research, and others are being validated, showing that this molecular biology tool can provide both researchers and clinicians with precious information concerning the behavior of tumors. Knowledge of the biochemical principles underlying this biotechnology can be of great value to interpret correctly qrt-PCR data.
引用
收藏
页码:189 / 195
页数:7
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