Analysis of 1-hydroxypyrene in urine as PAH exposure marker using in-situ derivatisation stir bar sorptive extraction-thermal desorption - Capillary gas chromatography - Mass spectrometry

被引：23

作者：

Desmet, K ^{[1
]}

Tienpont, B ^{[1
]}

Sandra, P ^{[1
]}

机构：

[1] State Univ Ghent, Organ Chem Lab, B-9000 Ghent, Belgium

来源：

CHROMATOGRAPHIA | 2003年 / 57卷 / 9-10期

关键词：

capillary gas chromatography - mass spectrometry; thermal desorption; stir bar sorptive extraction; in-situ derivatisation; 1-hydroxypyrene;

D O I：

10.1007/BF02491749

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fast and robust method for the determination of 1-hydroxypyrene (OH-pyrene) and other hydroxylated PAHs in urine by stir bar sorptive extraction (SBSE) combined with thermal desorption - capillary gas chromatography - mass spectrometry (TD-CGC-MS) is described. After enzymatic hydrolysis, in-situ derivatisation with acetic acid anhydride precedes SBSE sampling to improve the chromatographic behavior of the analytes. The performance of the method has been evaluated by the quantitative determination of OH-pyrene in some certified samples. Detection limits as low as 2 ng L-1 (ppt) can be obtained. The procedure is illustrated with the analysis of a urine sample of a firefighter.

引用

页码：681 / 685

页数：5

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