Assembly of single chromatin fibers depends on the tension in the DNA molecule: Magnetic tweezers study

被引：68

作者：

Leuba, SH

Karymov, MA

Tomschik, M

Ramjit, R

Smith, P

Zlatanova, J ^{[1
]}

机构：

[1] Univ Pittsburgh, Canc Inst Res Pavilion, Hillman Canc Ctr, Sch Med,Dept Cell Biol & Physiol, Pittsburgh, PA 15213 USA

[2] NCI, NIH, Bethesda, MD 20892 USA

[3] NIH, Div Bioengn Phys Sci, Off Res Serv, Bethesda, MD 20892 USA

[4] Polytech Univ, Dept Chem & Chem Engn, Brooklyn, NY 11201 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2003年 / 100卷 / 02期

关键词：

D O I：

10.1073/pnas.0136890100

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have used magnetic tweezers to study in real time chaperonemediated chromatin assembly/disassembly at the level of single chromatin fibers. We find a strong dependence of the rate of assembly on the exerted force, with strong inhibition of assembly at forces exceeding 10 pN. During assembly, and especially at higher forces, occasional abrupt increases in the length of the fiber were observed, giving a clear indication of reversibility of the assembly process. This result is a clear demonstration of the dynamic equilibrium between nucleosome assembly and disassembly at the single chromatin fiber level.

引用

页码：495 / 500

页数：6

共 39 条

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