Positional cloning in arabidopsis. Why it feels good to have a genome initiative working for you

被引：390

作者：

Lukowitz, W

Gillmor, CS

Scheible, WR

机构：

[1] Carnegie Inst Sci, Dept Plant Biol, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA

来源：

PLANT PHYSIOLOGY | 2000年 / 123卷 / 03期

关键词：

D O I：

10.1104/pp.123.3.795

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Positional (or map-based) cloning techniques are widely used to identify the protein products of genes defined by mutation. In Arabidopsis the information generated by the Genome Initiative is giving this approach a decisive boost. A wealth of sequence polymorphisms and molecular markers is now available and can be exploited for fine mapping with technically simple and robust polymerase chain reaction-based methods. As a result it has become possible to complete positional cloning projects in a short time and with relatively little effort.

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页码：795 / 805

页数：11

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