BIAcore for macromolecular interaction

被引：170

作者：

Fivash, M

Towler, EM

Fisher, RJ

机构：

[1] NCI, Frederick Canc Res & Dev Ctr, Data Management Serv Inc, SAIC, Frederick, MD 21702 USA

[2] NCI, Frederick Canc Res & Dev Ctr, Prot Chem Lab, SAIC, Frederick, MD 21702 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1998年 / 9卷 / 01期

关键词：

D O I：

10.1016/S0958-1669(98)80091-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Examination of the literature for the period of this review revealed nearly two hundred citations that employed surface plasmon resonance (SPR) spectroscopy using BIAcore technology to evaluate biospecific interactions, demonstrating the increasing popularity of this powerful technique. Among these we noted the development of several new applications/modifications of standard techniques. In general, we find the qualitative aspects of the reported experiments to be excellent but the quantitative descriptions (k(T), k(on), k(off), and K-eq) as well as the binding models still lagging behind. (C) Current Biology Ltd ISSN 0958-1669.

引用

页码：97 / 101

页数：5

共 34 条

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