Immunoglobulin E antibodies of atopic individuals exhibit a broad usage of VH-gene families

被引:19
作者
Eibensteiner, P
Spitzauer, S
Steinberger, P
Kraft, D
Valenta, R
机构
[1] Univ Vienna, Vienna Gen Hosp, Sch Med, Dept Pathophysiol,Mol Immunopathol Grp, A-1090 Vienna, Austria
[2] Univ Vienna, Vienna Gen Hosp, Inst Med & Chem Lab Diagnost, A-1090 Vienna, Austria
关键词
D O I
10.1046/j.1365-2567.2000.00078.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The term 'atopy' describes the genetically determined tendency to mount immunoglobulin E (IgE) antibody responses against per se harmless antigens (allergens). In this study we investigated the usage of V-H families in the formation of IgE antibodies in 10 patients suffering from mucosal and/or skin manifestations of atopy. IgE antibody reactivities to exogenous allergen sources as well as to autoallergens were determined and, by immunoabsorption, it was demonstrated that allergen-specific IgE accounted for most of the total serum IgE levels in these patients. Using primers with specificity for the V(H)1-6 gene families and a primer specific for the first constant region of human IgE, cDNAs coding for IgE heavy chain fragments were amplified using the reverse transcription-polymerase chain reaction (RT-PCR) from peripheral blood lymphocytes of the 10 atopic individuals. Hybridization of the heavy chain-encoding cDNAs with an IgE-specific internal oligonucleotide probe revealed a broad usage of all V-H-gene families in the atopic individuals. The spectrum of V-H families used in a given atopic individual was neither associated with the type or severity of clinical symptoms nor with the number of allergens recognized. The fact that allergen-specific IgE antibodies in atopic individuals originate from a broad variety of B cells thus reflects the activation of multiple B-cell clones during allergen sensitization. This finding should be borne in mind if therapeutic strategies for Type I allergy are considered that aim at a clonal elimination of allergen-specific B cells.
引用
收藏
页码:112 / 119
页数:8
相关论文
共 36 条
[1]   RESTRICTED IMMUNOGLOBULIN VH USAGE AND VDJ COMBINATIONS IN THE HUMAN RESPONSE TO HAEMOPHILUS-INFLUENZAE TYPE-B CAPSULAR POLYSACCHARIDE - NUCLEOTIDE-SEQUENCES OF MONOSPECIFIC ANTI-HAEMOPHILUS ANTIBODIES AND POLYSPECIFIC ANTIBODIES CROSS-REACTING WITH SELF-ANTIGENS [J].
ADDERSON, EE ;
SHACKELFORD, PG ;
QUINN, A ;
WILSON, PM ;
CUNNINGHAM, MW ;
INSEL, RA ;
CARROLL, WL .
JOURNAL OF CLINICAL INVESTIGATION, 1993, 91 (06) :2734-2743
[2]   The human antibody repertoire: Heavy and light chain variable region gene usage in six alloantibodies specific for human HLA class I and class II alloantigens [J].
Andris, JS ;
Abraham, SR ;
Pascual, V ;
Pistillo, MP ;
Mantero, S ;
Ferrara, GB ;
Capra, JD .
MOLECULAR IMMUNOLOGY, 1995, 32 (14-15) :1105-1122
[3]  
[Anonymous], ACTA DERM VENERE S92, DOI [10.2340/00015555924447, DOI 10.2340/00015555924447]
[4]  
CASOLARO V, 1996, CURR OPIN IMMUNOL, V9, P788
[5]  
Davis LG., 1986, Basic Methods in Molecular Biology
[6]   Molecular characterization of human IgG monoclonal antibodies specific for the major birch pollen allergen Bet v 1.: Anti-allergen IgG can enhance the anaphylactic reaction [J].
Denépoux, S ;
Eibensteiner, PB ;
Steinberger, P ;
Vrtala, S ;
Visco, V ;
Weyer, A ;
Kraft, D ;
Banchereau, J ;
Valenta, R ;
Lebecque, S .
FEBS LETTERS, 2000, 465 (01) :39-46
[7]   EFFECTS OF IL-4 AND IL-13 ON TOTAL AND ALLERGEN-SPECIFIC IGE PRODUCTION BY CULTURED PBMC FROM ALLERGIC PATIENTS DETERMINED WITH RECOMBINANT POLLEN ALLERGENS [J].
DOLECEK, C ;
STEINBERGER, P ;
SUSANI, M ;
KRAFT, D ;
VALENTA, R ;
BOLTZNITULESCU, G .
CLINICAL AND EXPERIMENTAL ALLERGY, 1995, 25 (09) :879-889
[8]  
FLICKER S, 1997, ALLERGY, V52, P11
[9]   IMMUNOGLOBULIN-E ANTIBODIES TO POLLEN ALLERGENS ACCOUNT FOR HIGH PERCENTAGES OF TOTAL IMMUNOGLOBULIN-E PROTEIN [J].
GLEICH, GJ ;
JACOB, GL .
SCIENCE, 1975, 190 (4219) :1106-1108
[10]  
IKEMATSU H, 1993, J IMMUNOL, V150, P1325