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Parathyroid hormone activation of the 25-hydroxyvitamin D3-1α-hydroxylase gene promoter

被引：182
作者：
Brenza, HL
Kimmel-Jehan, C
Jehan, F
Shinki, T
Wakino, S
Anazawa, H
Suda, T
DeLuca, HF
机构：
[1] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA
[2] Showa Univ, Sch Dent, Dept Biochem, Shinagawa Ku, Tokyo 142, Japan
[3] Keio Univ, Dept Internal Med, Shinjuku Ku, Tokyo 160, Japan
[4] Kyowa Hakko Kogyo Co Ltd, Tokyo Res Labs, Tokyo 194, Japan
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 04期
关键词：
vitamin D; calcium; bone; kidney; cyclic AMP;
D O I：
10.1073/pnas.95.4.1387
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The DNA flanking the 5' sequence of the mouse 1 alpha-hydroxylase gene has been cloned and sequenced. A TATA box has been located at -30 bp and aCCAAT box has been located at -79 bp. The gene's promoter activity has br!en demonstrated by using a luciferase reporter gene construct transfected into a modified pig kidney cell line, AOK-B50. Parathyroid hormone stimulates this promoter-directed synthesis of luciferase by 17-fold, whereas forskolin stimulates it by 3-fold. The action of parathyroid hormone is concentration-dependent. 1,25-Dihydroxyvitamin D-3 does not suppress basal promoter activity and marginally suppresses parathyroid hormone-driven luciferase reporter activity. The promoter has three potential cAMP-responsive element sites, and two perfect and one imperfect AP-1 sites, while no DR-3 was detected. These results indicate that parathyroid hormone stimulates 25-hydroxyvitamin D-3-1 alpha-hydroxylase by acting on the promoter of the 1 alpha-hydroxylase gene.
引用
收藏
页码：1387 / 1391
页数：5
相关论文
共 29 条
[1]   FORSKOLIN INCREASES 1,25-DIHYDROXYVITAMIN-D3 PRODUCTION BY RAT RENAL SLICES INVITRO [J].
ARMBRECHT, HJ ;
FORTE, LR ;
WONGSURAWAT, N ;
ZENSER, TV ;
DAVIS, BB .
ENDOCRINOLOGY, 1984, 114 (02) :644-649
[2]  
AUFFRAY C, 1980, EUR J BIOCHEM, V107, P303
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]  
BREATHNACH R, 1981, ANNU REV BIOCHEM, V50, P349, DOI 10.1146/annurev.bi.50.070181.002025
[5]   CLONED, STABLY EXPRESSED PARATHYROID-HORMONE (PTH)/PTH-RELATED PEPTIDE RECEPTORS ACTIVATE MULTIPLE MESSENGER SIGNALS AND BIOLOGICAL RESPONSES IN LLC-PK1 KIDNEY-CELLS [J].
BRINGHURST, FR ;
JUPPNER, H ;
GUO, J ;
URENA, P ;
POTTS, JT ;
KRONENBERG, HM ;
ABOUSAMRA, AB ;
SEGRE, GV .
ENDOCRINOLOGY, 1993, 132 (05) :2090-2098
[6]  
Darwish Hisham, 1993, Critical Reviews in Eukaryotic Gene Expression, V3, P89
[7]  
DELUCA HF, 1981, HARVEY LECT, V75, P333
[8]  
DEUTSCH PJ, 1988, J BIOL CHEM, V263, P18466
[9]   PATHOGENESIS OF HEREDITARY VITAMIN-D-DEPENDENT RICKETS - INBORN ERROR OF VITAMIN-D METABOLISM INVOLVING DEFECTIVE CONVERSION OF 25-HYDROXYVITAMIN-D TO 1ALPHA,25-DIHYDROXYVITAMIN-D [J].
FRASER, D ;
KOOH, SW ;
KIND, HP ;
HOLICK, MF ;
TANAKA, Y ;
DELUCA, HF .
NEW ENGLAND JOURNAL OF MEDICINE, 1973, 289 (16) :817-822
[10]   CONTROL OF 25-HYDROXYCHOLECALCIFEROL METABOLISM BY PARATHYROID GLANDS [J].
GARABEDIAN, M ;
BOYLE, IT ;
HOLICK, MF ;
DELUCA, HF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (07) :1673-+
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