Effect of culture temperature on a recombinant CHO cell line producing a C-terminal α-amidating enzyme

In order to seek an efficient method for producing a recombinant protein by using animal cell culture, we investigated various effects of the culture temperature on a recombinant CHO cell line (3 mu-1S), producing a C-terminal alpha-amidating enzyme (799BglII alpha-AE) originating from Xenopus laevis. The results revealed that a low culture temperature (below 37 degrees C) led to the following phenomena: [1] inhibited cell growth, [2] enhanced cellular productivity of the recombinant protein, [3] maintained high cell viability, [4] suppressed medium consumption, and [5] suppressed release of impurities from the cells. These findings indicate that a quite simple method, the culture at low temperature, will contribute to the total improvement of the industrial process for the production of the recombinant protein, 799BglII alpha-AE.