Interaction of photosystem 2-LHC2 supercomplexes in adjacent layers of stacked chloroplast thylakoid membranes

被引：11

作者：

Bumba, L

Husák, M

Vácha, F

机构：

[1] Univ S Bohemia, Fac Biol Sci, Ceske Budejovice 37005, Czech Republic

[2] Acad Sci Czech Republ, Inst Plant Mol Biol, CR-37005 Ceske Budejovice, Czech Republic

[3] Univ S Bohemia, Inst Phys Biol, Ceske Budejovice 37005, Czech Republic

来源：

PHOTOSYNTHETICA | 2004年 / 42卷 / 02期

关键词：

electron microscopy; membrane proteins; model; photosynthesis; photosystem; 2; Pisum; single particle image analysis;

D O I：

10.1023/B:PHOT.0000040590.10919.02

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Two types of photosystem 2-light-harvesting complex 2 (PS2-LHC2) supercomplexes with similar pigment and protein composition were isolated directly from thylakoid membranes by sucrose density gradient centrifugation. Electron microscopy and single particle image analysis revealed the first type as single unpaired PS2-LHC2 supercomplexes, whereas the second type was characterized as pairs of two PS2-LHC2 supercomplexes attached together by their stromal sides. Unstacking of thylakoid membranes resulted in a spontaneous disintegration of the paired supercomplexes into single unpaired particles. A model of the organisation of the pigment-protein complexes in grana region is proposed.

引用

收藏

页码：193 / 199

页数：7

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