Removal of proteoglycans from the surface of defects in articular cartilage transiently enhances coverage by repair cells

被引:107
作者
Hunziker, EB [1 ]
Kapfinger, E [1 ]
机构
[1] Univ Bern, ME Muller Inst Biomech, CH-3010 Bern, Switzerland
来源
JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME | 1998年 / 80B卷 / 01期
关键词
D O I
10.1302/0301-620X.80B1.7531
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Lesions within the articular cartilage layer of synovial joints do not heal spontaneously. Some repair cells may appear, but their failure to become established may be related to problems of adhesion to proteoglycan-rich surfaces. We therefore investigated whether controlled enzymatic degradation of surface proteoglycan molecules to a depth of about 1 mu m, using chondroitinase ABC, would improve coverage by repair cells. We created superficial lesions (1.0 X 0.2 X 5 mm) in the articular cartilage of mature rabbit knees and treated the surfaces with 1 U/ml of chondroitinase ABC for four minutes. The defects were studied by histomorphometry and electron microscopy at one, three and six months. At one month, untreated lesions were covered to a mean extent of 28% by repair cells; this was enhanced to a mean of 53% after enzyme treatment. By three months, the mean coverage of both control and chondroitinase-ABC-treated defects had diminished dramatically to 0.2% and 13%, respectively, but at six months both untreated and treated lesions had a similar coverage of about 30%, not significantly different from that achieved in untreated knees at one month. These findings suggest that, with time, chondrocytes near the surface of the defect may compensate for the loss of proteoglycans produced by enzyme treatment, thereby restoring the inhibitory properties of the matrix as regards cell adhesion. This supposition was confirmed by electron microscopy. Our results have an important bearing on attempts made to induce healing responses by transplanting chondrogenic cells or by applying growth factors.
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页码:144 / 150
页数:7
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