Differential binding characteristics of native monomeric and polymeric immunoglobulin A1 (IgA1) on human mesangial cells and the influence of in vitro deglycosylation of IgA1 molecules

被引：16

作者：

Gao, Y.-H. ^{[1
]}

Xu, L.-X. ^{[1
]}

Zhang, J.-J. ^{[1
]}

Zhang, Y. ^{[1
]}

Zhao, M.-H. ^{[1
]}

Wang, H.-Y. ^{[1
]}

机构：

[1] Peking Univ, Key Lab Renal Dis,Inst Nephrol, Minist Hlth China,Peking Univ Hosp 1, Renal Div,Dept Med, Beijing 100034, Peoples R China

来源：

CLINICAL AND EXPERIMENTAL IMMUNOLOGY | 2007年 / 148卷 / 03期

关键词：

binding capacity; deglycosylation; galactose; glycosylation; IgA nephropathy; IgA1; mesangial cell; radioligand; sialic acid;

D O I：

10.1111/j.1365-2249.2007.03374.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Recent studies had demonstrated that serum and mesangial immunoglobulin A1 (IgA1) in patients with IgA nephropathy (IgAN) were polymeric and deglycosylated. The current study was to investigate the binding characteristics of monomeric and polymeric normal human IgA1 on mesangial cells and the influence of in vitro deglycosylation of IgA1 molecules. The normal human IgA1 was desialylated and degalactosylated with specific enzymes, respectively. The monomeric IgA1 (mIgA1) and polymeric IgA1 (pIgA1) were separated by Sephacryl S-300 chromatography. The binding capacities of the mIgA1 and pIgA1 to primary human mesangial cells (HMC) were evaluated by classical radioligand assay. Both the native mIgA1 and pIgA1 could bind to HMC in a dose-dependent and saturable manner. The maximal binding capacity of the native pIgA1 were significantly higher than that of the native mIgA1 (P < 0.05). However, the affinity of the native mIgA1 was almost 100 times higher than that of the native pIgA1. After deglycosylation, binding of the two deglycosylated mIgA1 to HMC could not be detected. However, the maximal binding capacities of the two deglycosylated pIgA1 to HMC were increased significantly compared with that of native pIgA1. The affinity of the two deglycosylated pIgA1 was similar to that of native pIgA1 (P > 0.05). The current study suggests differential binding characteristics of native monomeric and polymeric IgA1 on mesangial cells. Glycosylation of IgA1 molecules could significantly affect the binding of IgA1 on HMC.

引用

页码：507 / 514

页数：8

共 39 条

[1] Mesangial IgA1 in IgA nephropathy exhibits aberrant O-glycosylation: Observations in three patients [J].