Single stranded DNA as an immunocytochemical marker for apoptotic change of ischemia in the gerbil hippocampus

被引：42

作者：

Maeda, M

Sugiyama, T

Akai, F

Jikihara, I

Hayashi, Y

Takagi, H

机构：

[1] Osaka City Univ, Sch Med, Dept Anat 1, Abeno Ku, Osaka 545, Japan

[2] Akita Univ, Sch Med, Dept Biochem 1, Akita 010, Japan

[3] Kinki Univ, Sch Med, Dept Neurosurg, Osaka 589, Japan

来源：

NEUROSCIENCE LETTERS | 1998年 / 240卷 / 02期

关键词：

single stranded DNA; apoptosis; hippocampus; ischemia; immunocytochemistry; gerbils;

D O I：

10.1016/S0304-3940(97)00901-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The light and electron microscopic localizations of single stranded DNA (SSD) protein, a marker of apoptosis and programmed cell death, in the gerbil hippocampus were examined by immunocytochemistry after transient brain ischemia. SSD-immunoreactive (IR) cells appeared from post-operative day 1 (PO 1) to PO 7 after 5- or 10-min ischemia. Immunoreaction was recognized in the nucleus of the CA1 pyramidal neurons without remarkable morphological changes on PO 1. These findings suggest that SSD degradation can occur during delayed neuronal death in the CA1, preceding the appearance of double strand breaks, one of the characteristic features of apoptosis. (C) 1998 Elsevier Science Ireland Ltd.

引用

收藏

页码：69 / 72

页数：4

相关论文

共 12 条

[1] P53 BINDS SINGLE-STRANDED-DNA ENDS THROUGH THE C-TERMINAL DOMAIN AND INTERNAL DNA SEGMENTS VIA THE MIDDLE DOMAIN [J].

BAKALKIN, G ;

SELIVANOVA, G ;

YAKOVLEVA, T ;

KISELEVA, E ;

KASHUBA, E ;

MAGNUSSON, KP ;

SZEKELY, L ;

KLEIN, G ;

TERENIUS, L ;

WIMAN, KG .

NUCLEIC ACIDS RESEARCH, 1995, 23 (03) :362-369

[2] P53 BINDS SINGLE-STRANDED-DNA ENDS AND CATALYZES DNA RENATURATION AND STRAND TRANSFER [J].

BAKALKIN, G ;

YAKOVLEVA, T ;

SELIVANOVA, G ;

MAGNUSSON, KP ;

SZEKELY, L ;

KISELEVA, E ;

KLEIN, G ;

TERENIUS, L ;

WIMAN, KG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (01) :413-417

[3] ANISOMYCIN AND CYCLOHEXIMIDE PROTECT CEREBELLAR NEURONS IN CULTURE FROM ANOXIA [J].

DESSI, F ;

CHARRIAUTMARLANGUE, C ;

BENARI, Y .

BRAIN RESEARCH, 1992, 581 (02) :323-326

[4]

Didier M, 1996, J NEUROSCI, V16, P2238

[5] EFFECTS OF CYCLOHEXIMIDE ON DELAYED NEURONAL DEATH IN RAT HIPPOCAMPUS [J].

GOTO, K ;

ISHIGE, A ;

SEKIGUCHI, K ;

IZUKA, S ;

SUGIMOTO, A ;

YUZURIHARA, M ;

ABURADA, M ;

HOSOYA, E ;

KOGURE, K .

BRAIN RESEARCH, 1990, 534 (1-2) :299-302

[6] DELAYED NEURONAL DEATH IN THE GERBIL HIPPOCAMPUS FOLLOWING ISCHEMIA [J].

KIRINO, T .

BRAIN RESEARCH, 1982, 239 (01) :57-69

[7] P53-IMMUNOREACTIVE PROTEIN AND P53 MESSENGER-RNA EXPRESSION AFTER TRANSIENT MIDDLE CEREBRAL-ARTERY OCCLUSION IN RATS [J].

LI, Y ;

CHOPP, M ;

ZHANG, ZG ;

ZALOGA, C ;

NIEWENHUIS, L ;

GAUTAM, S .

STROKE, 1994, 25 (04) :849-856

[8] GLOBAL-ISCHEMIA CAN CAUSE DNA FRAGMENTATION INDICATIVE OF APOPTOSIS IN RAT-BRAIN [J].

MACMANUS, JP ;

BUCHAN, AM ;

HILL, IE ;

RASQUINHA, I ;

PRESTON, E .

NEUROSCIENCE LETTERS, 1993, 164 (1-2) :89-92

[9] ANTIBODY AGAINST SINGLE-STRANDED-DNA DETECTS BOTH PROGRAMMED CELL-DEATH AND DRUG-INDUCED APOPTOSIS [J].

NARUSE, I ;

KEINO, H ;

KAWARADA, Y .

HISTOCHEMISTRY, 1994, 101 (01) :73-78

[10] INTERNUCLEOSOMAL DNA CLEAVAGE INVOLVED IN ISCHEMIA-INDUCED NEURONAL DEATH [J].

OKAMOTO, M ;

MATSUMOTO, M ;

OHTSUKI, T ;

TAGUCHI, A ;

MIKOSHIBA, K ;

YANAGIHARA, T ;

KAMADA, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 196 (03) :1356-1362