A study of biochemical and functional interactions of Htl1p, a putative component of the Saccharomyces cerevisiae, Rsc chromatin-remodeling complex

被引:5
作者
Florio, Carolina [1 ]
Moscariello, Mario [1 ]
Ederle, Sara [1 ]
Fasano, Rossella [1 ]
Lanzuolo, Chiara [1 ]
Pulitzer, John F. [1 ]
机构
[1] Univ Naples Federico II, Dipartimento Biol, I-80126 Naples, Italy
关键词
silencing; hydroxyurea; Rsc8; SIR3;
D O I
10.1016/j.gene.2007.02.002
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
HTL1, a small gene of Saccharomyces cerevisiae, encodes a 78-aminoacid peptide that influences the performance of a wide range of cellular processes [Lanzuolo, C., Ederle, S., Pollice, A., Russo, F., Storlazzi, A., Pulitzer, J.F., 200 1. The HTL1 gene,YCR020W-b of Saccharomyces cerevisiae is necessary for growth at 37 degrees C, and for the conservation of chromosome stability and fertility. Yeast, 18, 1317-1330]. Genetic interactions and co-immumoprecipitation experiments indicate a role for Hil1p in functions controlled by RSC, a multiprotein, ATP-dependent, chromatin-remodeling complex [Lu, Y.M., Lin, Y.R., Tsai, A., Hsao, YS., Li, C.C., Cheng, NLY., 2003. Dissecting the petl 8 mutation in Saccharomyces cercvisiae: HTLI encodes a 7-kDa polypeptide that interacts with components of the RSC complex. Mol. Genet. Genomics., 269, 321-330] [Romeo, M.J., Angus-Hill, M.L., Sobering, A.K., Kamada, Y, Cairns, 13R., Levin, D.E., 2002. HTL1 encodes a novel factor that interacts with the K c chromatin-remodeling complex in Saccharomyces cerevisiae. Mol. Cell. Biol., 22, 8165-8174]. Htl1p and RSC components, share the property of associating with TBP a component of general multiprotem transcription factor TFIID [Sanders, S.L., Jennings, J., Camitescu, A., Link, A.J., Weil, P.A., 2002. Proteomics of the eukaryotic transcription machinery: identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry. Mol. Cell. Biol. 22, 4723-4738]. We confirm, by integrating genetic and biochemical experiments, that Htl1p binding to the RSC complex is direct and physiologically relevant and show that it is mediated by Rsc8p, a core component of the RSC complex. Deletion of HTL1, Re depletion ofRSC core subunits [Moreira, J.M., Holmberg, S., 1999. Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex Rsc. Embo J., 18, 2836-2844], leads to constitutive transcription of the CHA1 locus. This transcriptional phenotype exhibits variable penetrance. Deletion of HTL1 also leads to hydroxyarea hypersensitivity at 30 degrees C, suggesting a defect in replication/repair. This defect leads, during cell growth, to selection of mutations at the SJR3 locus that suppress hydroxyurea sensitivity. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:72 / 85
页数:14
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