Pseudomonas koreensis sp nov., Pseudomonas umsongensis sp nov and Pseudomonas jinjuensis sp nov., novel species from farm soils in Korea

Among Pseudomonas strains isolated from Korean agricultural soils, four strains (Ps 9-14 group: Ps 1-2, Ps 1-10, Ps 5-5 and Ps 9-14(T)) from the Suwon, Goesan and Samchok regions, three strains (Ps 3-10 group: Ps 2-22, Ps 3-1 and Ps 3-1 Or) from Umsong Region and four strains (Pss 26 group: Pss 14, Pss 25, Pss 26(T) and Pss 27) from Jinju Region were identified as three independent groups on the basis of 16S rDNA sequence analysis. While, on the basis of 16S rDNA sequence analysis, Ps 9-14(T) and Ps 3-1 Or form a phyletic line with Pseudomonas jessenii CIP 105274(T), 'Pseudomonas pavonaceae' IAM 1155 and Pseudomonas graminis DSM 11363 T, pSS 26(T) is grouped with Pseudomonas citronellolis ATCC 13674(T) and Pseudomonas nitroreducens IAM 1439(T). According to DNA-DNA hybridization studies, strain Ps 9-14(T) shows high DNA relatedness to strain Ps 3-10(T) (52 %) and Pseudomonas migulae CIP 105470(T) (49 %) and strain Ps 3-10(T) reveals high relatedness to strain Ps 9-14 (48 %) and P. jessenii CIP 105274 (45 %). Strain Pss 26(T) shows high relatedness to P. citronellolis LMG 18378(T) (54%), P. nitroreducens ATCC 33634(T) (48 %) and Pseudomonas aeruginosa LMG 1242(T) (48 %). On the basis of. phenotypic and genotypic analyses, three novel species of the genus Pseudomonas are proposed: Pseudomonas koreensis sp. nov. (type strain Ps 9-14(T) =LMG 213 1 8 T =KACC 10848(T)) for the Ps 9-14 group, Pseudomonas umsongensis sp. nov. (type strain Ps 3-1 Or =LMG 21318(T) =KACC 10847(T)) for the Ps 3-10 group and Pseudomonas jinjuensis sp. nov. (type strain Pss 26(T) =LMG 21316(T) =KACC 10760(T)) for the Pss 26 group.