Attachment and phospholipase A2-induced lysis of phospholipid bilayer vesicles to plasma-polymerized maleic anhydride/SiO2 multilayers

被引：11

作者：

Chifen, Anye N.

Foerch, Renate

Knoll, Wolfgang

Cameron, Petra J.

Khor, Hwei L.

Williams, Thomas L.

Jenkins, A. Toby A. ^{[1
]}

机构：

[1] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

[2] Max Planck Inst Polymer Res, D-55128 Mainz, Germany

来源：

LANGMUIR | 2007年 / 23卷 / 11期

关键词：

D O I：

10.1021/la0701273

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This article describes a method by which intact vesicles can be chemically attached to hydrolyzed maleic anhydride films covalently bound to plasma-polymerized SiO2 on Au substrates. Surface plasmon field-enhanced fluorescence spectroscopy (SPFS) combined with surface plasmon resonance spectroscopy (SPR) was used to monitor the activation of plasma-deposited maleic anhydride (pp-MA) film with EDC/NHS and the subsequent coupling of lipid vesicles. The vesicles were formed from a mixture of phosphatidylcholine and phosphatidylethanolamine lipids, with a water-soluble fluorophore encapsulated within. Vesicle attachment was measured in real time on plasma films formed under different pulse conditions (plasma duty cycle). Optimum vesicle attachment was observed on the pp-MA films containing the highest density of maleic anhydride groups. Phospholipase A(2) was used to lyse the surface-bound vesicles and to release the encapsulated fluorophore.

引用

收藏

页码：6294 / 6298

页数：5

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