Clinical implication of plasma neutrophil gelatinase-associated lipocalin (NGAL) concentrations in patients with advanced carotid atherosclerosis

被引:34
作者
Giaginis, Constantinos [1 ]
Zira, Athina [1 ]
Katsargyris, Athanasios [2 ]
Klonaris, Chris [2 ]
Theocharis, Stamatios [1 ]
机构
[1] Univ Athens, Sch Med, Dept Forens Med & Toxicol, GR-11527 Athens, Greece
[2] Univ Athens, Sch Med, Laikon Hosp, Dept Surg 1, GR-11527 Athens, Greece
关键词
atherosclerosis; carotid; medical history; NGAL; risk factors; LEUKOCYTE ACTIVATION; INFLAMMATORY MEDIATORS; IMMUNE-RESPONSE; EXPRESSION; PROTEIN; INJURY; IRON;
D O I
10.1515/CCLM.2010.211
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: Neutrophil gelatinase-associated lipocalin (NGAL) is well established as an early and specific biomarker of kidney disease. Recent evidence further suggests that NGAL may play a crucial role in vascular remodeling and plaque instability during the development of atherosclerosis. Methods: Plasma NGAL concentrations measured using a solid-phase enzyme-linked immunosorbent assay (ELISA) were correlated with medical history, risk factors and medication intake in 141 patients with advanced carotid atherosclerotic lesions who underwent carotid endarterectomy for vascular repair. Results: Plasma NGAL concentrations were associated with patient age (R-s=0.2055, p=0.0144), plasma homocysteine (R-s=0.4274, p<0.00001) and serum creatinine (R-s=0.4640, p<0.00001) concentrations and estimated glomerular filtration rate (eGFR) (R-s=-0.4911, p<0.00001). Hypertensive patients, as well as those receiving therapy with angiotensin converting enzyme (ACE) inhibitors, presented with significantly enhanced plasma NGAL concentrations when compared to normotensive (p=0.0341) patients and those not treated (p=0.0004). Enhanced NGAL concentrations did not meet statistical significance for patients with advanced stenosis grade (p=0.0971) or a history of peripheral artery disease (p=0.0827). Multiple regression analysis identified homocysteine, creatinine, eGFR and treatment with ACE inhibitors (p=0.0019, -0.00001, 0.0005 and 0.0219, respectively) as independent predictors of NGAL concentration. Conclusions: Plasma NGAL concentrations were associated with patient age, hypertension, eGFR, creatinine and homocysteine concentrations and therapy with ACE inhibitors. The role of NGAL in the development of atherosclerosis needs to be further explored taking into consideration the uncontrolled effect of renal disease in atherosclerotic patients with multiple risk factors. Clin Chem Lab Med 2010; 48: 1035-41.
引用
收藏
页码:1035 / 1041
页数:7
相关论文
共 33 条
[1]   Lipocalins:: unity in diversity [J].
Åkerstrom, B ;
Flower, DR ;
Salier, JP .
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2000, 1482 (1-2) :1-8
[2]   Increasing levels of leukocyte-derived inflammatory mediators in plasma and cAMP in platelets during follow-up after acute cerebral ischemia [J].
Anwaar, I ;
Gottsäter, A ;
Ohlsson, K ;
Mattiasson, I ;
Lindgärde, F .
CEREBROVASCULAR DISEASES, 1998, 8 (06) :310-317
[3]   Neutrophil gelatinase-associated lipocalin (NGAL) as a marker of kidney damage [J].
Bolignano, Davide ;
Donato, Valentina ;
Coppolino, Giuseppe ;
Campo, Susanna ;
Buemi, Antoine ;
Lacquaniti, Antonio ;
Buemi, Michele .
AMERICAN JOURNAL OF KIDNEY DISEASES, 2008, 52 (03) :595-605
[4]   The metabolic syndrome and cardiovascular disease [J].
Bonora, E .
ANNALS OF MEDICINE, 2006, 38 (01) :64-80
[5]   Induction of neutrophil gelatinase-associated lipocalin in vascular injury via activation of nuclear factor-κB [J].
Bu, De-xiu ;
Hemdahl, Anne-Louise ;
Gabrielsen, Anders ;
Fuxe, Jonas ;
Zhu, Chaoyong ;
Eriksson, Per ;
Yan, Zhong-qun .
AMERICAN JOURNAL OF PATHOLOGY, 2006, 169 (06) :2245-2253
[6]   Homocysteine metabolism, hyperhomocysteinaemia and vascular disease: An overview [J].
Castro, R ;
Rivera, I ;
Blom, HJ ;
Jakobs, C ;
de Almeida, IT .
JOURNAL OF INHERITED METABOLIC DISEASE, 2006, 29 (01) :3-20
[7]   Neutrophil gelatinase-associated lipocalin (NGAL): A new marker of kidney disease [J].
Devarajan, Peasad .
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 2008, 68 :89-94
[8]   Leukocyte activation in atherosclerosis: Correlation with risk factors [J].
Elneihoum, AM ;
Falke, P ;
Hedblad, B ;
Lindgarde, F ;
Ohlsson, K .
ATHEROSCLEROSIS, 1997, 131 (01) :79-84
[9]   Leukocyte activation detected by increased plasma levels of inflammatory mediators in patients with ischemic cerebrovascular diseases [J].
Elneihoum, AM ;
Falke, P ;
Axelsson, L ;
Lundberg, E ;
Lindgarde, F ;
Ohlsson, K .
STROKE, 1996, 27 (10) :1734-1738
[10]   Leukocyte activation: Relation to cardiovascular mortality after cerebrovascular ischemia [J].
Falke, P ;
Elneihoum, AM ;
Ohlsson, K .
CEREBROVASCULAR DISEASES, 2000, 10 (02) :97-101