Capillary zone electrophoresis of some organic acids in milk whey

被引：9

作者：

Buiarelli, F ^{[1
]}

Cartoni, G ^{[1
]}

Coccioli, F ^{[1
]}

Jasionowska, R ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

来源：

JOURNAL OF SEPARATION SCIENCE | 2003年 / 26卷 / 05期

关键词：

capillary zone electrophoresis; CZE; milk analysis; orotic acid;

D O I：

10.1002/jssc.200390055

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper describes a method for analysing some acids of milk whey by Capillary Zone Electrophoresis. After eliminating the whey proteins by ultrafiltration, the whey underwent electrophoretic separation in the presence of anodic electroosmotic flow. The following analytes were detected: citric, orotic, uric, and hippuric acids. A procedure is described for sample preparation and the operating conditions for electrophoretic capillary separation established. Finally, orotic acid is quantitatively determined.

引用

页码：425 / 428

页数：4

共 11 条

[1] ISOLATION, QUANTIFICATION, AND BIOSYNTHETIC ORIGIN OF OROTIC-ACID IN MILK [J].

AHMED, AA ;

PORTER, GA ;

MCCARTHY, RD .

JOURNAL OF DAIRY SCIENCE, 1978, 61 (01) :39-43

[2] Orotic acid: a milk constituent - Enzymatic determination by means of a new microcalorimetric method [J].

Anastasi, G ;

Antonelli, ML ;

Biondi, A ;

Vinci, G .

TALANTA, 2000, 52 (05) :947-952

[3]

[Anonymous], 1990, ITAL J FOOD SCI

[4] Indirect ultraviolet detection of biologically relevant organic acids by capillary electrophoresis [J].

Chen, H ;

Xu, Y ;

VanLente, F ;

Ip, MPC .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1996, 679 (1-2) :49-59

[5] APPLICATION OF MICELLAR ELECTROKINETIC CHROMATOGRAPHY AND INDIRECT UV DETECTION FOR THE ANALYSIS OF FATTY-ACIDS [J].

ERIM, FB ;

XU, X ;

KRAAK, JC .

JOURNAL OF CHROMATOGRAPHY A, 1995, 694 (02) :471-479

[6] Development of a CE method to analyze organic acids in dairy products:: Application to study the metabolism of heat-shocked spores [J].

Izco, JM ;

Tormo, M ;

Jiménez-Flores, R .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (07) :1765-1773

[7] BOVINE-MILK OROTIC-ACID - VARIABILITY AND SIGNIFICANCE FOR HUMAN-NUTRITION [J].

ROBINSON, JL .

JOURNAL OF DAIRY SCIENCE, 1980, 63 (05) :865-871

[8] ANALYSIS AND STABILITY OF OROTIC-ACID IN MILK [J].

SAIDI, B ;

WARTHESEN, JJ .

JOURNAL OF DAIRY SCIENCE, 1989, 72 (11) :2900-2905

[9]

STRYER L, 1988, BIOCHEMISTRY-US, P607

[10]

Theobald A, 1996, HRC-J HIGH RES CHROM, V19, P608

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