Isolation and characterization of polymorphic microsatellite loci in Acanthoscelides obvelatus Bridwell (Coleoptera: Bruchidae)

被引:8
作者
Alvarez, N [1 ]
Aebi, A
Risterucci, AM
Hossaert-Mckey, M
Benrey, B
机构
[1] Univ Neuchatel, Inst Zool, LEAE, CH-2007 Neuchatel, Switzerland
[2] CNRS, CEFE, F-34293 Montpellier 5, France
[3] CIRAD, AMIS, BIOTROP, Montpellier, France
来源
MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 01期
关键词
Acanthoscelides obvelatus; Bruchidae; Coleoptera; microsatellite; pest species; Phaseolus;
D O I
10.1046/j.1471-8286.2003.00335.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Five microsatellite loci were isolated from the bruchid Acanthoscelides obvelatus Bridwell (Coleoptera: Bruchidae). Each locus was polymorphic, with the number of alleles ranging from two to 15. We found high levels of within-population variation at most loci, with heterozygosity ranging from 0.182 to 0.900. Cross-species amplification of these loci was tested in two other species of the genus Acanthoscelides, A. obtectus Say and A. argillaceus Sharp.
引用
收藏
页码:12 / 14
页数:3
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