Enhanced galactosyltransferase expression in the failing hearts of spontaneously hypertensive rats

被引：3

作者：

Humphries, DE ^{[1
]}

Sirokman, G ^{[1
]}

Bing, OHL ^{[1
]}

机构：

[1] BOSTON UNIV,SCH MED,DEPT MED,BOSTON,MA 02118

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 218卷 / 01期

关键词：

D O I：

10.1006/bbrc.1996.0056

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

To examine differences in cardiac gene expression between spontaneously hypertensive rats with and without heart failure, we have used subtractive hybridization to identify differentially expressed genes. After subtraction, cDNAs were amplified by PCR, cloned and sequenced. One of 36 independent cDNAs was found to be 86% homologous to murine UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase RNA blot analysis confirmed the similar to 4.0 kb rat galactosyltransferase transcript was increased in failing hearts relative to non-failing hearts. Biochemical assay also showed increased galactosyltransferase activity in the failing hearts. (C) 1996 Academic Press, Inc.

引用

页码：320 / 324

页数：5

共 17 条

[1]

BEGOVAC PC, 1994, J BIOL CHEM, V269, P31793

[2]

THE SPONTANEOUSLY HYPERTENSIVE RAT AS A MODEL OF THE TRANSITION FROM COMPENSATED LEFT-VENTRICULAR HYPERTROPHY TO FAILURE [J].