Antifreeze proteins bind independently to ice

被引：80

作者：

DeLuca, CI ^{[1
]}

Comley, R ^{[1
]}

Davies, PL ^{[1
]}

机构：

[1] Queens Univ, Dept Biochem, Kingston, ON K7L 3N6, Canada

来源：

BIOPHYSICAL JOURNAL | 1998年 / 74卷 / 03期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/S0006-3495(98)77862-2

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

It has been suggested that cooperative interactions between antifreeze proteins (AFPs) on the ice surfaces are required for complete inhibition of ice crystal growth. To test this hypothesis, a 7-kDa type III AFP was linked through its N-terminus to thioredoxin (12 kDa) or maltose-binding protein (42 kDa). The resultant 20-kDa and 50-kDa fusion proteins were larger in diameter than free AFP and thus precluded any extensive AFP-AFP contacts on the ice surface. Both fusion proteins were at least as active as free AFP at virtually all concentrations tested. By these criteria, AFPs function independently of each other and do not require specific intermolecular interactions to bind tightly to ice.

引用

页码：1502 / 1508

页数：7

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