A real-time PCR assay for the detection of azoxystrobin-resistant Alternaria populations from pistachio orchards in California

被引：24

作者：

Ma, ZH ^{[1
]}

Michailides, TJ ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Plant Pathol, Kearney Agr Ctr, Parlier, CA 93648 USA

来源：

CROP PROTECTION | 2004年 / 23卷 / 12期

关键词：

Alternaria late blight of pistachio; QoI fungicides; real-time PCR;

D O I：

10.1016/j.cropro.2004.05.012

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Azoxystrobin-resistant populations of Alternaria spp. in the alternata, tenuissima, and arborescens species-groups, the causal agents of Alternaria late blight of pistachio, have been selected in pistachio orchards in California. The azoxystrobin resistance in Alternaria spp. was found to be correlative to a single point mutation resulting in the replacement of a glycine by an alanine at codon 143 (G143A) in the mitochondrial cytochrome b (cyt b) gene. Based on this point mutation, a real-time PCR method was developed to rapidly detect azoxystrobin-resistant Alternaria populations in field leaf samples collected from pistachio orchards in California. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：1259 / 1263

页数：5

共 5 条

[1] The strobilurin fungicides [J].

Bartlett, DW ;

Clough, JM ;

Godwin, JR ;

Hall, AA ;

Hamer, M ;

Parr-Dobrzanski, B .

PEST MANAGEMENT SCIENCE, 2002, 58 (07) :649-662

[2] Following the dynamics of strobilurin resistance in Blumeria graminis f.sp tritici using quantitative allele-specific real-time PCR measurements with the fluorescent dye SYBR Green I [J].

Fraaije, BA ;

Butters, JA ;

Coelho, JM ;

Jones, DR ;

Hollomon, DW .

PLANT PATHOLOGY, 2002, 51 (01) :45-54

[3] An allele-specific PCR assay for detecting azoxystrobin-resistant Alternaria isolates from pistachio in California [J].

Ma, Z ;

Michailides, TJ .

JOURNAL OF PHYTOPATHOLOGY, 2004, 152 (02) :118-121

[4] Resistance to azoxystrobin in Alternaria isolates from pistachio in California [J].

Ma, ZH ;

Felts, D ;

Michailides, TJ .

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2003, 77 (02) :66-74

[5] Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with Alternaria late blight of pistachio [J].

Pryor, BM ;

Michailides, TJ .

PHYTOPATHOLOGY, 2002, 92 (04) :406-416

← 1 →