Brevundimonas mediterranea sp nov., a non-stalked species from the Mediterranean Sea

Six strains of Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from the Mediterranean Sea. 16S rRNA gene sequence analysis indicated that the strains were affiliated within the alphaproteobacterial genus Brevundimonas, with Brevundimonas intetmedia (99(.)4%) and Brevundimonas vesicularis (99(.)2%) as their closest relatives. This affiliation was supported by chemotaxonomic data (major polar lipids: phosphatidyl diacylglycerol, sulfoquinovosyl diacylglycerol and phosphaticlyl glucopyranosyl diacylglycerol; major fatty acids: C-18:1, C-16:0, C-16:1, C-15:0, C(17:1)omega8c, 11-Me-C(18:1)omega5t). The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the strains from all recognized Brevundimonas species. The strains therefore represent a novel species, for which the name Brevundimonas mediterranea sp. nov. is proposed, with the type strain V4.BO.10(T) (= LMG 21911(T) = CIP 107934(T)).