Expression and function of Toll-like receptor 2 in canine blood phagocytes

被引：18

作者：

Bazzocchi, C ^{[1
]}

Mortarino, M ^{[1
]}

Comazzi, S ^{[1
]}

Bandi, C ^{[1
]}

Franceschi, A ^{[1
]}

Genchi, C ^{[1
]}

机构：

[1] Univ Milan, Sez Patol Gen & Parasitol, Dipartimento Patol Anim Igiene & Sanita Publ Vet, I-20133 Milan, Italy

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2005年 / 104卷 / 1-2期

关键词：

innate immunity; Toll-like receptor 2; canine PMNs; IL-8; expression; flow cytometry;

D O I：

10.1016/j.vetimm.2004.08.023

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Toll-like receptors (TLRs) are a family of highly conserved pattern recognition receptors (PRR) of mammals that participate in the activation of innate immune responses against microbial infections. Among these receptors, TLR2 is essential for the recognition of conserved structural components of bacteria, protozoa and fungi. Until now, expression of TLR2 in dogs has not been investigated. In this work we describe a partial sequence of the gene coding for canine TLR2 and show that TLR2 mRNA is constitutively expressed in canine blood PMNs. We also show that stimulation of purified PMNs with lipoteichoic acid (LTA), a ligand of TLR2, leads to the release of proinflammatory chemokine IL-8. Furthermore, TLR2 protein is easily detectable by flow cytometry on the canine peripheral blood granulocyte and monocyte cell surface, and slightly on lymphocytes. These findings suggest that, also in dogs as in humans the initial antibacterial response of PMNs could be elicited through engagement of TLR2. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：15 / 19

页数：5

共 16 条

[1] Cutting edge: Cell surface expression and lipopolysaccharide signaling via the Toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages [J].