HPLC analysis of vitamin E isoforms in human epidermis: Correlation with minimal erythema dose and free radical scavenging activity

被引:30
作者
Fuchs, J
Weber, S
Podda, M
Groth, N
Herrling, T
Packer, L
Kaufmann, R
机构
[1] Univ Frankfurt, Sch Med, Dept Dermatol, Frankfurt, Germany
[2] Univ Bonn, Sch Med, Dept Anesthesiol & Intens Care Med, D-5300 Bonn, Germany
[3] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[4] Tech Univ Berlin, Berlin, Germany
[5] Univ So Calif, Sch Pharm, Dept Mol Pharmacol & Toxicol, Los Angeles, CA 90033 USA
关键词
free radicals; antioxidant; redox; tocotrienols; EPR; skin;
D O I
10.1016/S0891-5849(02)01293-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The content and composition of different vitamin E isoforms was analyzed in normal human skin. Interestingly the epidermis contained 1% alpha-tocotrienol, 3% gamma-tocotrienol, 87% alpha-tocopherol, and 9% gamma-tocopherol. Although the levels of tocotrienol in human epidermis appear to be considerably lower than reported in the hairless mouse, the presence of significant amounts of tocotrienol levels leads to speculation about the physiological function of tocotrienols in skin. Besides antioxidant activity and photoprotection, tocotrienols may have skin barrier and growth-modulating properties. A good correlation was found for epidermal alpha-tocopherol (r = 0.7909, p < .0003), gamma-tocopherol (r = 0.556, p < .025), and the total vitamin E content (r = 0.831, p < .0001) with the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging in epidermis, as assessed by electron paramagnetic resonance (EPR) spectroscopy. In human epidermis, alpha-tocopherol is quantitatively the most important vitamin E isoform present and comprises the bulk of first line free radical defense in the lipid compartment. Epidermal tocotrienol levels were not correlated with DPPH scavenging activity. The minimal erythema dose (MED), an individual measure for sun sensitivity and a crude indicator for skin cancer susceptibility, did not correlate with the epidermal content of the vitamin E isoforms. Hence it is concluded that vitamin E alone is not a determinant of individual photosensitivity in humans. (C) 2003 Elsevier Science Inc.
引用
收藏
页码:330 / 336
页数:7
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