Single-strand conformation polymorphism (SSCP) analysis of Listeria monocytogenes iap gene as tool to detect different serogroups

被引：14

作者：

Manzano, M

Cocolin, L

Pipan, C

Falasca, E

Botta, GA

Cantoni, C

Comi, G

机构：

[1] Univ Udine, Dipartimento Sci Alimenti, Fac Agr, I-33100 Udine, Italy

[2] Univ Udine, Fac Med & Chirurg, Ist Microbiol, I-33100 Udine, Italy

[3] Univ Milan, Fac Vet Med, Ist Ispez Alimenti Origine Anim, Milan, Italy

来源：

MOLECULAR AND CELLULAR PROBES | 1997年 / 11卷 / 06期

关键词：

Listeria monocytogenes; polymerase chain reaction (PCR); single-strand conformation polymorphism (SSCP); iap gene;

D O I：

10.1006/mcpr.1997.0137

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

PCR-single-strand conformation polymorphism (PCR-SSCP) analysis is a convenient technique for the detection of mutations. As the mobility of single-stranded DNA is sequence-dependent it could therefore be used to determine serotype-related sequence variations in Listeria monocytogenes. Sero-specific patterns were observed in different L. monocytogenes serogroups. (C) 1997 Academic Press Limited.

引用

页码：459 / 462

页数：4

共 24 条

[1] PULSED-FIELD FINGERPRINTING OF LISTERIAE - IDENTIFICATION OF GENOMIC DIVISIONS FOR LISTERIA-MONOCYTOGENES AND THEIR CORRELATION WITH SEROVAR [J].