Fusion of the Endoplasmic Reticulum and Mitochondrial Outer Membrane in Rats Brown Adipose Tissue: Activation of Thermogenesis by Ca2+

被引:37
作者
de Meis, Leopoldo [1 ]
Ketzer, Luisa A. [1 ]
da Costa, Rodrigo Madeiro [1 ]
de Andrade, Ivone Rosa [2 ]
Benchimol, Marlene [2 ]
机构
[1] Univ Fed Rio de Janeiro, Ctr Ciencias Saude, Inst Bioquim Med, Rio De Janeiro, Brazil
[2] Univ Santa Ursula, Lab Ultraestrutura Celular, Rio De Janeiro, Brazil
来源
PLOS ONE | 2010年 / 5卷 / 03期
关键词
HEAT-PRODUCTION; CA2+-ATPASE; ATPASE; ENERGY; WATER; IDENTIFICATION; TEMPERATURE; HYDROLYSIS; LIVER;
D O I
10.1371/journal.pone.0009439
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Brown adipose tissue (BAT) mitochondria thermogenesis is regulated by uncoupling protein 1 (UCP 1), GDP and fatty acids. In this report, we observed fusion of the endoplasmic reticulum (ER) membrane with the mitochondrial outer membrane of rats BAT. Ca2+-ATPase (SERCA 1) was identified by immunoelectron microscopy in both ER and mitochondria. This finding led us to test the Ca2+ effect in BAT mitochondria thermogenesis. We found that Ca2+ increased the rate of respiration and heat production measured with a microcalorimeter both in coupled and uncoupled mitochondria, but had no effect on the rate of ATP synthesis. The Ca2+ concentration needed for half-maximal activation varied between 0.08 and 0.11 mu M. The activation of respiration was less pronounced than that of heat production. Heat production and ATP synthesis were inhibited by rotenone and KCN. Liver mitochondria have no UCP1 and during respiration synthesize a large amount of ATP, produce little heat, GDP had no effect on mitochondria coupling, Ca2+ strongly inhibited ATP synthesis and had little or no effect on the small amount of heat released. These finding indicate that Ca2+ activation of thermogenesis may be a specific feature of BAT mitochondria not found in other mitochondria such as liver.
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页数:9
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