Genetic diversity and identification of cymbidium cultivars as measured by random amplified polymorphic DNA (RAPD) markers

被引:68
作者
Obara-Okeyo, P [1 ]
Kako, S [1 ]
机构
[1] Yamaguchi Univ, Fac Agr, Lab Vegetable & Ornamental Hort, Yamaguchi 753, Japan
关键词
cluster analysis; Cymbidium; genetic diversity; RAPD markers;
D O I
10.1023/A:1018374226074
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
DNA from thirty-six cymbidium cultivars was examined using polymerase chain reaction (PCR) to determine the efficiency of randomly amplified polymorphic DNA (RAPD) markers in identifying cultivars and determining levels of genetic variability. A total of 132 RAPD markers, 78% of which were polymorphic, were produced from 15 10mer arbitrary primers. All the cultivars were distinguishable when a number of primers was considered. One cultivar, Blue Smoke 'Green Meadow' could be distinguished from all the rest based only on lack of the OPA5-370 fragment. Genetic distances among the cultivars were estimated based on the amount of band sharing and ranged from 0.08-0.50 with an average of 0.29. Cluster analysis of genetic distance estimates grouped siblings together with each other and parents with offsprings, thereby agreeing with known parentage information and corroborating isozyme data obtained from a separate study. The possible application of the observed polymorphism and variation to cymbidium breeding is discussed.
引用
收藏
页码:95 / 101
页数:7
相关论文
共 18 条