I-menthol-induced [Ca2+]i increase and impulses in cultured sensory neurons

被引：37

作者：

Okazawa, M ^{[1
]}

Terauchi, T ^{[1
]}

Shiraki, T ^{[1
]}

Matsumura, K ^{[1
]}

Kobayashi, S ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Informat, Dept Intelligence Sci & Technol, Div Biol Informat, Kyoto 6068501, Japan

来源：

NEUROREPORT | 2000年 / 11卷 / 10期

关键词：

Ca2+](i); dorsal root ganglion; Fura-2; I-menthol; microfluorimetry; patch-clamp recording;

D O I：

10.1097/00001756-200007140-00018

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We investigated the effects of l-menthol on cultured dorsal root ganglion (DRG) cells, instead of free nerve endings of sensory fibers. Using Fura-2 microfluorimetry, we identified a few DRG neurons that showed an increase in intracellular free Ca2+ concentration ([Ca2+](i)) in response to l-menthol. They made up only 10% of the neurons activated by a high K+ solution. l-Menthol induced the [Ca2+](i) increase in a dose-dependent manner, with an EC50 of 37.9 mu M and a Hill coefficient of 0.97. A related compound, cyclohexanol, had no effect. When extracellular Ca2+ was removed, l-menthol did not induce the [Ca2+](i) increase. Whole-cell current-clamp recordings revealed that l-menthol induced depolarization (13.2 mV, receptor potential) leading to impulses. We conclude that l-menthol induced the impulses through activation of menthol receptors in a small subset of the cultured sensory neurons. NeuroReport 11:2151-2155 (C) 2000 Lippincott Williams & Wilkins.

引用

页码：2151 / 2155

页数：5

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