Analyses of peripheral blood mononuclear cells in operational tolerance after pediatric living donor liver transplantation

被引:221
作者
Li, Y
Koshiba, T [1 ]
Yoshizawa, A
Yonekawa, Y
Masuda, K
Ito, A
Ueda, M
Mori, T
Kawamoto, H
Tanaka, Y
Sakaguchi, S
Minato, N
Wood, KJ
Tanaka, K
机构
[1] Kyoto Univ, Fac Med, Dept Transplantat & Immunol, Kyoto, Japan
[2] Kyoto Univ, Fac Med, Horizontal Med Res Org, Transplant Tolerance Unit, Kyoto, Japan
[3] Daigo Watanbe Clin, Kyoto 6011375, Japan
[4] Kyoto Univ, Grad Sch Biostudies, RIKEN RCAI, Lab Lymphocyte Dev, Kyoto, Japan
[5] Kyoto Univ, Lab Immunol & Cell Biol, Kyoto, Japan
[6] Kyoto Univ, Inst Frontier Med Sci, Dept Expt Pathol, Kyoto, Japan
[7] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Surg, Oxford OX3 9DU, England
关键词
FACS analysis; liver transplantation; living-related liver donors; lymphocytes; tolerance;
D O I
10.1111/j.1600-6143.2004.00611.x
中图分类号
R61 [外科手术学];
学科分类号
摘要
Operational tolerance (graft acceptance in an immunosuppression (IS)-free environment) after living-donor liver transplantation (LDLT) could occur by our elective protocol in some patients. There is, nevertheless, no reliable parameter to monitor patients who may discontinue IS without a risk of rejection. To identify such parameters, we systemically phenotyped peripheral blood mononuclear cells from operationally tolerant patients. An increase was observed in the frequency of CD4 + CD25(high+) cells, B cells and Vdelta1/Vdelta2 gammadelta T-cells ratio in operationally tolerant patients (Gr-tol; n = 12), compared with those from age-matched volunteers (Gr-vol; n = 24) or patients on IS (Gr-IS; n = 19). The frequency of NK cells was decreased in Gr-tol, compared with those in Gr-IS or Gr-vol. The frequency of NKT cells was decreased after LDLT, compared with that in Gr-vol. Although the contribution of those subsets to the tolerant state remains elusive, the results may provide important clues for reliable indicators of tolerance after LDLT.
引用
收藏
页码:2118 / 2125
页数:8
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