Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin

被引：61

作者：

Reymond, N

Borg, JP

Lecocq, E

Adelaide, J

Campadelli-Fiume, G

Dubreuil, P

Lopez, M

机构：

[1] INSERM, U119, Unite Cancerol & Therapeut Expt, F-13009 Marseille, France

[2] Inst J Paoli I Calmettes, F-13009 Marseille, France

[3] Univ Bologna, Dept Expt Pathol, I-40126 Bologna, Italy

来源：

GENE | 2000年 / 255卷 / 02期

关键词：

immunoglobulin superfamily; receptor; adhesion; herpes virus; nectin; afadin;

D O I：

10.1016/S0378-1119(00)00316-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have isolated nectin3/PRR3, the fourth human member of the nectin/PRR family, also described as the alpha herpes virus receptor family. Nectin/PRR members are adhesion molecules expressed at intercellular junctions. Nectin3/PRR3 is a transmembrane protein, whose extracellular region contains three Ig-like domains (V, C and C) and shares approximately 30% identity with the other members. It is mainly expressed in testis and placental tissues. SDS-PAGE analyses demonstrate that nectin3/PRR3 has a molecular weight of 83 kDa. Nectin1/PRR1L and nectin2/PRR2S and L were found to be specifically expressed at the intercellular junctions. This localization is in part due to the interaction of the C-terminal part of these receptors (ended by the consensus sequence A/EXYV) and the PDZ domain of afadin. In this report we demonstrate that the nectin3/PRR3 receptor carries the A/EXYV consensus sequence and interacts in vivo with both long and short isoforms of afadin. These results suggest that the human nectin3/PRR3 is a new afadin-associated molecule. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：347 / 355

页数：9

共 30 条

[1]

AOKI J, 1994, J BIOL CHEM, V269, P8431

[2]

CAMPADELLI FG, 2000, IN PRESS REV MED VIR

[3] The V domain of herpesvirus Ig-like receptor (HIgR) contains a major functional region in herpes simplex virus-1 entry into cells and interacts physically with the viral glycoprotein D [J].