Cloning and analysis of expression profile of 13 WRKY genes in rice

被引：58

作者：

Qiu, YP

Jing, SJ

Fu, J

Li, L

Yu, DQ ^{[1
]}

机构：

[1] Chinese Acad Sci, Kunming Sect, Xishuangbanna Trop Bot Garden, Kunming 650223, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 2004年 / 49卷 / 20期

基金：

中国国家自然科学基金;

关键词：

rice; transcriptional factor WRKY genes; abiotic stress factors; gene expression profiles;

D O I：

10.1360/982004-183

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The transcriptional factor WRKY proteins contain the highly conserved amino acid sequence WRKYGQK as well as the novel zinc-finger-like motifs Cys(2)His(2) or Cys(2)HisCys. A search of the rice genome identified 97 genes encoding WRKY proteins. Of these 97 WRKY homologs found in rice, 13 cDNAs encoding WRKY proteins were consequently isolated from a rice cDNA library constructed from 4degreesC-treated shoots by probing for the conserved WRKY domain. Northern blotting analysis revealed that 10 of 13 OsWRKY genes were differentially regulated in plants that were treated by the four following abiotic stress factors: NaCl, PEG, cold (4degreesC) and heat (42degreesC). The resulting expression profiles exhibited great differences in both the manner and timing of their response to the four different abiotic treatments. The difference of gene expression profiles suggested the different physiological functions among the WRKY genes.

引用

收藏

页码：2159 / 2168

页数：10

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