Identification and biological evaluation of grapefruit oil components as potential novel efflux pump modulators in methicillin-resistant Staphylococcus aureus bacterial strains

被引：42

作者：

Abulrob, AN ^{[1
]}

Suller, MTE ^{[1
]}

Gumbleton, M ^{[1
]}

Simons, C ^{[1
]}

Russell, AD ^{[1
]}

机构：

[1] Cardiff Univ, Welsh Sch Pharm, Cardiff CF10 3XF, S Glam, Wales

来源：

PHYTOCHEMISTRY | 2004年 / 65卷 / 22期

关键词：

grapefruit oil; efflux pump modulators; 4-{[(E)-5-(3,3-dimethyl-2-oxiranyl)-3-methyl-2-pentenyl]oxy}-7H-furo[3,2-g]chromen-7-one (bergamottin epoxide); MRSA and MSSA strains;

D O I：

10.1016/j.phytochem.2004.08.044

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methicillin-resistant Staphylococcus aureus (MRSA) and MSSA strains were treated with: (a) grapefruit oil (GFO) components, isolated by chromatography and characterised by NMR and mass spectroscopy; (b) antimicrobial agents, or (c) a combination of both to evaluate (MIC determination) intrinsic antibacterial activity and to determine whether GFO components could modulate bacterial sensitivity to the anti-bacterial agents. Preliminary data suggested that the grapefruit component 4-{[(E)-5-(3,3-dimethyl-2-oxiranyl)-3-methyl-2-pentenyl]oxy}-7H-furo[3,2-g]chromen-7-one (2) enhances the susceptibility of test MRSA strains to agents, e.g., ethidium bromide and norfloxacin, to which these micro-organisms are normally resistant. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：3021 / 3027

页数：7

共 12 条

[1]

ABULROB AG, 2001, Patent No. 0100689

[2] The effects of NorA inhibition on the activities of levofloxacin, ciprofloxacin and norfloxacin against two genetically related strains of Staphylococcus aureus in an in-vitro infection model [J].

Aeschlimann, JR ;

Kaatz, GW ;

Rybak, MJ .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1999, 44 (03) :343-349

[3] CHEMOTAXONOMY OF RUTACEAE .9. COUMARINS OF CITRUS-MACROPTERA [J].

DREYER, DL ;

HUEY, PF .

PHYTOCHEMISTRY, 1973, 12 (12) :3011-3013

[4]

Herwaldt Loreen A., 1999, American Journal of Medicine, V106, p11S, DOI 10.1016/S0002-9343(98)00350-7

[5] Active efflux, a common mechanism for biocide and antibiotic resistance [J].

Levy, SB .

JOURNAL OF APPLIED MICROBIOLOGY, 2002, 92 :65S-71S

[6] FLUOROQUINOLONE RESISTANCE PROTEIN NORA OF STAPHYLOCOCCUS-AUREUS IS A MULTIDRUG EFFLUX TRANSPORTER [J].

NEYFAKH, AA ;

BORSCH, CM ;

KAATZ, GW .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1993, 37 (01) :128-129

[7] Proton-dependent multidrug efflux systems [J].

Paulsen, IT ;

Brown, MH ;

Skurray, RA .

MICROBIOLOGICAL REVIEWS, 1996, 60 (04) :575-+

[8] Efflux-mediated resistance to fluoroquinolones in gram-negative bacteria [J].

Poole, K .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (09) :2233-2241

[9]

SchmiedlinRen P, 1997, DRUG METAB DISPOS, V25, P1228

[10] The effect of reserpine, an inhibitor of multidrug efflux pumps, on the in-vitro activities of ciprofloxacin, sparfloxacin and moxifloxacin against clinical isolates of Staphylococcus aureus [J].

Schmitz, FJ ;

Fluit, AC ;

Lückefahr, M ;

Engler, B ;

Hofmann, B ;

Verhoef, J ;

Heinz, HP ;

Hadding, U ;

Jones, ME .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1998, 42 (06) :807-810

← 1 2 →