Influence of pr-M Cleavage on the Heterogeneity of Extracellular Dengue Virus Particles

被引：124

作者：

Junjhon, Jiraphan ^{[2
]}

Edwards, Thomas J. ^{[1
]}

Utaipat, Utaiwan ^{[3
]}

Bowman, Valorie D. ^{[1
]}

Holdaway, Heather A. ^{[1
]}

Zhang, Wei ^{[1
]}

Keelapang, Poonsook ^{[2
]}

Puttikhunt, Chunya ^{[4
]}

Perera, Rushika ^{[1
]}

Chipman, Paul R. ^{[1
]}

Kasinrerk, Watchara ^{[5
,6
]}

Malasit, Prida ^{[4
,7
]}

Kuhn, Richard J. ^{[1
]}

Sittisombut, Nopporn ^{[2
,4
]}

机构：

[1] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA

[2] Chiang Mai Univ, Fac Med, Dept Microbiol, Chiang Mai 50200, Thailand

[3] Chiang Mai Univ, Res Inst Hlth Sci, Chiang Mai 50200, Thailand

[4] Natl Sci & Technol Dev Agcy, Natl Ctr Genet Engn & Biotechnol, Med Biotechnol Unit, Bangkok 10400, Thailand

[5] Chiang Mai Univ, Fac Associated Med Sci, Dept Med Technol, Chiang Mai 50200, Thailand

[6] Natl Sci & Technol Dev Agcy, Natl Ctr Genet Engn & Biotechnol, Biomed Technol Res Ctr, Bangkok 10400, Thailand

[7] Mahidol Univ, Siriraj Hosp, Fac Med, Med Mol Biol Unit, Bangkok 10700, Thailand

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 16期

关键词：

BORNE ENCEPHALITIS-VIRUS; FLAVIVIRUS; INFECTION; ANTIBODIES; DOMAIN; CELLS; DETERMINANTS; GLYCOPROTEIN; RECOGNITION; ACTIVATION;

D O I：

10.1128/JVI.00696-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage.

引用

页码：8353 / 8358

页数：6

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