A quantitative competitive PCR assay for the covalently closed circular form of the duck hepatitis B virus

被引：42

作者：

Addison, WR

Wong, WWS

Fischer, KP

Tyrrell, DLJ

机构：

[1] Univ Alberta, Dept Med Microbiol & Immunol, Edmonton, AB T6G 2S2, Canada

[2] Univ Alberta, Glaxo Wellcome Heritage Res Inst, Edmonton, AB T6G 2S2, Canada

[3] Univ Alberta, Dept Med, Edmonton, AB T6G 2S2, Canada

来源：

ANTIVIRAL RESEARCH | 2000年 / 48卷 / 01期

关键词：

DHBV; cccDNA; liver; competitive PCR; hepadnavirus;

D O I：

10.1016/S0166-3542(00)00114-5

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A crucial step in the establishment and maintenance of a hepadnavirus infection is the formation of a pool of covalently closed circular vital genomes in the nucleus. Changes in the size of this pool occur when an infection is established, when acute infections are resolved, and when chronic infections are treated with antiviral drugs. However, the lack of a quantitative assay for the cccDNA form of the virus has hampered study of the biology of this replication intermediate. In response to this need we have devised a sensitive and accurate competitive PCR assay that is highly selective for the cccDNA form of the duck hepatitis B virus. Since only small amounts of DNA are needed for the assay, cccDNA pool sizes can he monitored in live animals using DNA derived from needle biopsies of infected liver. (C) 1000 Elsevier Science B.V. All rights reserved.

引用

页码：27 / 37

页数：11

共 23 条

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Ausubel F.M., 1992, CURRENT PROTOCOLS MO

[2]

Clementi M, 1993, PCR Methods Appl, V2, P191

[3] REVERSION OF DUCK HEPATITIS-B VIRUS-DNA REPLICATION IN-VIVO FOLLOWING CESSATION OF TREATMENT WITH THE NUCLEOSIDE ANALOG GANCICLOVIR [J].