Proteomics of the chloroplast: experimentation and prediction

被引：51

作者：

van Wijk, KJ ^{[1
]}

机构：

[1] Univ Stockholm, Arrhenius Labs Nat Sci, Dept Biochem, S-10691 Stockholm, Sweden

来源：

TRENDS IN PLANT SCIENCE | 2000年 / 5卷 / 10期

关键词：

D O I：

10.1016/S1360-1385(00)01737-4

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

New technologies, in combination with increasing amounts of plant genome sequence data, have opened up incredible experimental possibilities to identify the total set of chloroplast proteins (the chloroplast proteome) as well as their expression levels and post-translational modifications in a global manner. This is summarized under the term 'proteomics' and typically involves two-dimensional electrophoresis or chromatography, mass spectrometry and bioinformatics. Complemented with nucleotide-based global techniques, proteomics is expected to provide many new insights into chloroplast biogenesis, adaptation and function.

引用

页码：420 / 425

页数：6

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