Proteomics of the chloroplast: experimentation and prediction

被引:51
作者
van Wijk, KJ [1 ]
机构
[1] Univ Stockholm, Arrhenius Labs Nat Sci, Dept Biochem, S-10691 Stockholm, Sweden
关键词
D O I
10.1016/S1360-1385(00)01737-4
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
New technologies, in combination with increasing amounts of plant genome sequence data, have opened up incredible experimental possibilities to identify the total set of chloroplast proteins (the chloroplast proteome) as well as their expression levels and post-translational modifications in a global manner. This is summarized under the term 'proteomics' and typically involves two-dimensional electrophoresis or chromatography, mass spectrometry and bioinformatics. Complemented with nucleotide-based global techniques, proteomics is expected to provide many new insights into chloroplast biogenesis, adaptation and function.
引用
收藏
页码:420 / 425
页数:6
相关论文
共 62 条
[1]   A prediction of the size and evolutionary origin of the proteome of chloroplasts of Arabidopsis [J].
Abdallah, F ;
Salamini, F ;
Leister, D .
TRENDS IN PLANT SCIENCE, 2000, 5 (04) :141-142
[2]   Two-dimensional electrophoresis of membrane proteins: A current challenge for immobilized pH gradients [J].
Adessi, C ;
Miege, C ;
Albrieux, C ;
Rabilloud, T .
ELECTROPHORESIS, 1997, 18 (01) :127-135
[3]   The role of lipids in plastid protein transport [J].
Bruce, BD .
PLANT MOLECULAR BIOLOGY, 1998, 38 (1-2) :223-246
[4]  
Burlingame AL, 1998, ANAL CHEM, V70, p647R
[5]   Patterns of protein synthesis and tolerance of anoxia in root tips of maize seedlings acclimated to a low-oxygen environment, and identification of proteins by mass spectrometry [J].
Chang, WWP ;
Huang, L ;
Shen, M ;
Webster, C ;
Burlingame, AL ;
Roberts, JKM .
PLANT PHYSIOLOGY, 2000, 122 (02) :295-317
[6]  
Cordwell SJ, 2000, ELECTROPHORESIS, V21, P1094, DOI 10.1002/(SICI)1522-2683(20000401)21:6<1094::AID-ELPS1094>3.3.CO
[7]  
2-S
[8]  
Corthals GL, 2000, ELECTROPHORESIS, V21, P1104, DOI 10.1002/(SICI)1522-2683(20000401)21:6<1104::AID-ELPS1104>3.0.CO
[9]  
2-C
[10]   Probing protein function using a combination of gene knockout and proteome analysis by mass spectrometry [J].
Dainese, P ;
Staudenmann, W ;
Quadroni, M ;
Korostensky, C ;
Gonnet, G ;
Kertesz, M ;
James, P .
ELECTROPHORESIS, 1997, 18 (3-4) :432-442