P2X7 receptor expression levels determine lethal effects of a purine based danger signal in T lymphocytes

被引：46

作者：

Aswad, Fred

Dennert, Gunther ^{[1
]}

机构：

[1] Univ So Calif, Keck Sch Med, Norris Comprehens Canc Ctr, Dept Mol Microbiol, Los Angeles, CA 90033 USA

[2] Univ So Calif, Keck Sch Med, Norris Comprehens Canc Ctr, Dept Immunol, Los Angeles, CA 90033 USA

来源：

CELLULAR IMMUNOLOGY | 2006年 / 243卷 / 01期

关键词：

P2X(7); T cells; NAD; ATP; cell death;

D O I：

10.1016/j.cellimm.2006.12.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Contact of T lymphocytes with nicotinamide adenine dinucleotide (NAD) or ATP causes cell death that requires expression of purinergic receptor P2X(7) (P2X(7)R). T cell subsets differ in their responses to NAD and ATP, which awaits a mechanistic explanation. Here, we show that sensitivity to ATP correlates with P2X(7)R expression levels in CD4 cells, CD8 cells and CD4(+)CD25(+) cells from both C57BL/6 and BALB/c mice. But P2X(7)R ligands do not only induce cell death but also shedding of CD62L. It is shown here that in CD62L(high) T cells, CD62L shedding correlates with low expression of P2X(7)Rs and lower cell death, whereas in CD62L(low) cells P2X(7)R expression and death are higher. The possibility is therefore investigated that P2X(7)Rs induce T cell activation. Experiments show that spontaneous T cell proliferation is somewhat higher in cells expressing P2X(7)Rs, but this effect we suggest is caused by P2X7R expression on accessory cells. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：58 / 65

页数：8

共 23 条

[1] Basal activation of the P2X7 ATP receptor elevates mitochondrial calcium and potential, increases cellular ATP levels, and promotes serum-independent growth [J].