Wide mapping of a T-DNA insertion site in oilseed rape using bulk segregant analysis and comparative mapping

被引：3

作者：

Baranger, A ^{[1
]}

Delourme, R ^{[1
]}

Foisset, N ^{[1
]}

Eber, F ^{[1
]}

Barret, P ^{[1
]}

Dupuis, P ^{[1
]}

Renard, M ^{[1
]}

Chèvre, AM ^{[1
]}

机构：

[1] INRA, Stn Ameliorat Plantes, F-35653 Le Rheu, France

来源：

PLANT BREEDING | 1997年 / 116卷 / 06期

关键词：

Brassica napus; bulk segregant analysis; comparative mapping; doubled haploid lines; safety assessment; T-DNA insertion site;

D O I：

10.1111/j.1439-0523.1997.tb02189.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Doubled haploid oilseed rape lines segregating for a transgene inducing herbicide resistance (bar gene) were investigated for the wide mapping of the T-DNA insertion site. Bulk segregant analysis using presence/absence and intensity polymorphisms between the bulks, as well as comparative mapping with a linkage group deriving from another cross, led to the identification of 11 random amplified polymorphic DNA (RAPD) markers tightly or loosely linked to the bar gene. Ten RAPD loci out of 11 were located on the same side of the bar locus, strongly suggesting that the T-DNA integrated in a telomeric or subtelomeric position. The eleventh RAPD marker exhibited a strong segregation distortion, which could be the result of a heteroduplex formation. Comparison of the linkage groups obtained from the two crosses showed different recombination rates between markers, possibly reflecting differences in parental genetic backgrounds. Consequences and potential applications in transgene dispersal safety assessment studies are discussed.

引用

页码：553 / 560

页数：8

共 47 条

[1] LOCALIZATION OF AGROBACTERIUM-RHIZOGENES T-DNA IN PLANT CHROMOSOMES BY INSITU HYBRIDIZATION [J].