Detection of tau phosphorylated at threonine 231 in cerebrospinal fluid of Alzheimer's disease patients

被引：148

作者：

Kohnken, R

Buerger, K

Zinkowski, R

Miller, C

Kerkman, D

DeBernardis, J

Shen, JF

Möller, HJ

Davies, P

Hampel, H

机构：

[1] Mol Geriatr Corp, Vernon Hills, IL USA

[2] Univ Munich, Dementia Res Sect & Memory Clin, Dept Psychiat, Munich, Germany

[3] Neurol Consultants Montgomery PC, Montgomery, AL USA

[4] Albert Einstein Coll Med, Dept Pathol, Bronx, NY 10467 USA

来源：

NEUROSCIENCE LETTERS | 2000年 / 287卷 / 03期

关键词：

Alzheimer's disease; tau protein; cerebrospinal fluid; diagnosis; phosphotau;

D O I：

10.1016/S0304-3940(00)01178-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A new sandwich ELISA is described which shows specificity for tau phosphorylated at threonine 231 and preferentially reacts with Alzheimer's disease (AD) brain extracts relative to other dementias. This assay was used to analyze 58 antemortem cerebrospinal fluid samples. Twenty-three of 27 AD samples (85% sensitivity) yielded signals greater than the cutoff, while only one of 31 non-AD samples (97% specificity) were greater. This indicates that detection of phosphotau in cerebrospinal fluid with this sandwich ELISA could prove useful in the diagnosis of AD. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

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页码：187 / 190

页数：4

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