Absolute optical cross section of individual fluorescent molecules

被引：40

作者：

Kastrup, L ^{[1
]}

Hell, SW ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept Nanobiophoton, D-37077 Gottingen, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 48期

关键词：

D O I：

10.1002/anie.200461337

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Light switches: The photon capture area of single fluorescent molecules has been directly measured by switching them on and off" with light. Stimulated emission of single molecules (see picture) enables the precise control of the excited state population probability of an individual molecule at room temperature."

引用

页码：6646 / 6649

页数：4

共 21 条

[1]

Basche T., 1997, SINGLE MOL OPTICAL D

[2] SINGLE MOLECULES OBSERVED BY NEAR-FIELD SCANNING OPTICAL MICROSCOPY [J].

BETZIG, E ;

CHICHESTER, RJ .

SCIENCE, 1993, 262 (5138) :1422-1425

[3] On/off blinking and switching behaviour of single molecules of green fluorescent protein [J].

Dickson, RM ;

Cubitt, AB ;

Tsien, RY ;

Moerner, WE .

NATURE, 1997, 388 (6640) :355-358

[4] Fluorescence lifetime imaging by asynchronous pump-probe microscopy [J].

Dong, CY ;

So, PTC ;

French, T ;

Gratton, E .

BIOPHYSICAL JOURNAL, 1995, 69 (06) :2234-2242

[5] Photostability of a fluorescent marker under pulsed excited-state depletion through stimulated emission [J].

Dyba, M ;

Hell, SW .

APPLIED OPTICS, 2003, 42 (25) :5123-5129

[6] Photobleaching of fluorescent dyes under conditions used for single-molecule detection: Evidence of two-step photolysis [J].

Eggeling, C ;

Widengren, J ;

Rigler, R ;

Seidel, CAM .

ANALYTICAL CHEMISTRY, 1998, 70 (13) :2651-2659

[7]

Einstein A, 1917, PHYS Z, V18, P121

[8] Real-time light-driven dynamics of the fluorescence emission in single green fluorescent protein molecules [J].

Garcia-Parajo, MF ;

Segers-Nolten, GMJ ;

Veerman, JA ;

Greve, J ;

van Hulst, NF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (13) :7237-7242

[9] BREAKING THE DIFFRACTION RESOLUTION LIMIT BY STIMULATED-EMISSION - STIMULATED-EMISSION-DEPLETION FLUORESCENCE MICROSCOPY [J].

HELL, SW ;

WICHMANN, J .

OPTICS LETTERS, 1994, 19 (11) :780-782

[10]

HELL SW, 1995, J MICROSC-OXFORD, V180, pRPU

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