Overexpressed mortalin (mot-2)/mthsp70/GRP75 and hTERT cooperate to extend the in vitro lifespan of human fibroblasts

被引：82

作者：

Kaul, SC

Yaguchi, T

Taira, K

Reddel, RR

Wadhwa, R

机构：

[1] Natl Inst Adv Ind Sci & Technol, Res Ctr Glycosci, Tsukuba, Ibaraki 3058566, Japan

[2] Childrens Med Res Inst, Westmead, NSW 2145, Australia

来源：

EXPERIMENTAL CELL RESEARCH | 2003年 / 286卷 / 01期

关键词：

mortalin (mot-2)/mthsp70/GRP75; telomerase; human skin fibroblasts; lifespan extension; immortalization;

D O I：

10.1016/S0014-4827(03)00101-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The lifespan of human foreskin fibroblasts (HFF5), cultured under standard in vitro conditions (including ambient atmospheric oxygen tension), was extended slightly by expression of exogenous mortalin (mot-2)/mthsp70/Grp75, but not by the catalytic subunit of telomerase, hTERT. Together, mot-2 and hTERT permitted bypass of senescence, a substantial extension of lifespan, and possibly immortalization. This is the first demonstration that mot-2 and telomerase can cooperate in the immortalization process. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：96 / 101

页数：6

共 38 条

[1] Switching and signaling at the telomere [J].