Covalently coupling the antibody on an amine-self-assembled gold surface to probe hyaluronan-binding protein with capacitance measurement

被引：67

作者：

Jiang, DC

Tang, J

Liu, BH

Yang, PY

Shen, XR

Kong, JL ^{[1
]}

机构：

[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

[2] Naval Med Res Inst, Shanghai 200433, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2003年 / 18卷 / 09期

基金：

中国国家自然科学基金;

关键词：

hyaluronan-binding cartilage protein; capacitive immunosensor; self-assembled monolayers; amine-modified; potentiostatic-step;

D O I：

10.1016/S0956-5663(02)00253-1

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Hyaluronan-binding proteins (HABPs), the important structural components of extracellular matrices, served important structural and regulatory functions during development and in maintaining adult tissue homestats. A sensitive, specific and rapid-responsing immunosensor to probe hyaluronan-binding cartilage protein was presented in this work. The novel immunosensor supplied a label-free detection method for HABP, which was based on measuring the capacitance change in-between the unlabeled HABP (antigen) and rabbit-anti-HABP (Ra-HABP, antibody). The HABP immumosensor was prepared by covalently coupling Ra-HABP on an amine-self-assembled gold surface with glutaraldehyde. The capacitance change corresponding to the concentration of HABP, the target antigen, was evaluated by an electrochemical approach called potentiostatic-step in microseconds. The immunosensor showed a specific response to HABP in the range 10-1000 ng/ml. The presented work supplied a promising clinical screening method. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：1183 / 1191

页数：9

共 35 条

[1] Sensing of acetylcholine by a tricomponent-enzyme layered electrode using faradaic impedance spectroscopy, cyclic voltammetry, and microgravimetric quartz crystal microbalance transduction methods [J].